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Article|13 Dec 2023|OPEN
A Ca2+ sensor BraCBL1.2 involves in BraCRa-mediated clubroot resistance in Chinese cabbage 
Yinglan Piao1,5 , Shizhen Li2,5 , Yiduo Chen3 and Sisi Zhao1 , Zhongyun Piao4 , , Haiping Wang,1 ,
1State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China
2State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
3Institut für Biologie und Biotechnologie der Pf lanzen, Westfälische Wilhelms-Universität, Münster 48143, Germany
4College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China
5Yinglan Piao and Shizhen Li contributed equally to this work
*Corresponding author. E-mail: zypiao@syau.edu.cn,wanghaiping@caas.cn

Horticulture Research 11,
Article number: uhad261 (2024)
doi: https://doi.org/10.1093/hr/uhad261
Views: 25

Received: 09 Aug 2023
Accepted: 26 Nov 2023
Published online: 13 Dec 2023

Abstract

Clubroot disease caused by Plasmodiophora brassicae (P. brassicae) severely threatens the cultivation of Cruciferous plants, especially Chinese cabbage. Recently, resistance genes in plants have been reported to encode for a Ca2+-permeable channel in the plasma membrane, which can mediate the cytosolic Ca2+ increase in plant cells upon pathogen attack. However, the downstream Ca2+ sensor and decoder are still unknown. In this study, we identified the virulent and avirulent P. brassicae isolates (Pbs) of two near isogenic lines, CR 3–2 and CS 3–2, with CR 3–2 harboring clubroot resistant gene BraCRa. The transcriptomic analysis was then conducted with CR 3–2 after inoculating with virulent isolate PbE and avirulent isolate Pb4. From the differentially expressed genes of transcriptomic data, we identified a Ca2+-sensor encoding gene, BraCBL1.2, that was highly induced in CR 3–2 during infection by Pb4 but not by PbE. Moreover, GUS histochemical staining and subcellular localization analysis revealed that BraCBL1.2 was specifically expressed in the root hair cells of Arabidopsis and encoded a putative Ca2+ sensor localized in the plasma membrane. We also developed an assay to investigate the BraCRa-mediated hypersensitive response (HR) in tobacco leaves. The results suggest that BraCBL1.2 is involved in the BraCRa-mediated plant ETI immune response against P. brassicae. In addition, we verified that overexpression of BraCBL1.2 enhanced clubroot resistance in Arabidopsis. Collectively, our data identified the involvement of a Ca2+ sensor in BraCRa-mediated clubroot resistance in Chinese cabbage, providing a theoretical basis for further research on the resistance of Chinese cabbage to P. brassicae.