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Article|27 Nov 2023|OPEN
Construction of a high-density genetic map for yardlong bean and identification of ANT1 as a regulator of anthocyanin biosynthesis
Hongmei Zhang1 , ,† , Wei Zhang1 ,† , Shan Meng2 ,† , Linchong Hui3 , Xiaoqing Liu1 , Wei Chen3 , Wei Yan2 , Xin Chen1 and Huatao Chen,1 ,
1Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing 210014, China
2Institute of Germplasm Resources and Biotechnology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
3Lianyungang Institute of Agricultural Sciences, Jiangsu Academy of Agricultural Sciences, Lianyungang, 222000, China
*Corresponding author. E-mail: zhm@jaas.ac.cn,cht@jaas.ac.cn
Hongmei Zhang,Wei Zhang and Shan Meng contributed equally to the study.

Horticulture Research 11,
Article number: uhad247 (2024)
doi: https://doi.org/10.1093/hr/uhad247
Views: 20

Received: 17 Jul 2023
Accepted: 12 Nov 2023
Published online: 27 Nov 2023

Abstract

Because its long, tender pods supply essential proteins, vitamins, and fibers to humans, yardlong bean (Vigna unguiculata ssp. sesquipedalis) is a commonly consumed vegetable, especially in Southeast Asia. To provide insights into the genetic bases of key agricultural traits in yardlong bean, we here created a high-density bin-map with 2084 bin markers using 514 227 SNPs from a recombinant-inbred line (RIL) population. Quantitative trait loci (QTL) mapping was carried out to identify loci associated with anthocyanin content (ANT), vitamin E content (VE), total soluble protein content (TSP), pod length (PL), hundred-seed weight (HSW), seed length and width (SL and SW, respectively), and seed coat color (SCC). In total, 20 related QTLs were isolated, explaining 7.58–56.03% of the phenotypic variation. Of these, five major QTLs (qANT5qTSP11qVE7qPL3, and qSCC9) were detected in 2020, 2021, and the combined environment, explaining 11.96–56.03% of the phenotypic variation. VuANT1 was identified as a causal gene for the QTL qANT5, which regulated anthocyanin content; VuANT1 was highly expressed in immature purple pods but barely detectable in white pods. VuANT1 overexpression in tobacco leaves and yardlong bean hairy roots resulted in purple coloration as a result of anthocyanin accumulation. These findings suggested that VuANT1 was a key regulator of anthocyanin accumulation in yardlong bean. Our results lay a firm foundation for target agricultural trait improvement and clarification of the genetic mechanisms underlying agricultural traits in yardlong bean.