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Article|28 Nov 2023|OPEN
Signalling mechanisms and agricultural applications of (Z)-3-hexenyl butyrate-mediated stomatal closure
Celia Payá1 , Borja Belda-Palazón1 ,† , Francisco Vera-Sirera1 ,† , Julia Pérez-Pérez1 , Lucía Jordá2,3 , Ismael Rodrigo1 , José María Bellés1 and María Pilar López-Gresa1 , Purificación Lisón,1 ,
1Instituto de Biología Molecular y Celular de Plantas (IBMCP), Consejo Superior de Investigaciones Científicas (CSIC), Ciudad Politécnica de la Innovación (CPI) 8E, Universitat Politècnica de València (UPV), Ingeniero Fausto Elio s/n, 46011 Valencia, Spain
2Centro de Biotecnología y Genómica de Plantas (UPM-INIA), Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Universidad Politécnica de Madrid, Pozuelo de Alarcón, 28223 Madrid, Spain
3Departamento de Biotecnología-Biología Vegetal, Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas, Universidad Politécnica de Madrid, 28040 Madrid, Spain
*Corresponding author. E-mail: plison@ibmcp.upv.es
Both authors contributed equally to the study.

Horticulture Research 11,
Article number: uhad248 (2024)
doi: https://doi.org/10.1093/hr/uhad248
Views: 21

Received: 08 Aug 2023
Accepted: 12 Nov 2023
Published online: 28 Nov 2023

Abstract

Biotic and abiotic stresses can severely limit crop productivity. In response to drought, plants close stomata to prevent water loss. Furthermore, stomata are the main entry point for several pathogens. Therefore, the development of natural products to control stomata closure can be considered a sustainable strategy to cope with stresses in agriculture. Plants respond to different stresses by releasing volatile organic compounds. Green leaf volatiles, which are commonly produced across different plant species after tissue damage, comprise an important group within volatile organic compounds. Among them, (Z)-3-hexenyl butyrate (HB) was described as a natural inducer of stomatal closure, playing an important role in stomatal immunity, although its mechanism of action is still unknown. Through different genetic, pharmacological, and biochemical approaches, we here uncover that HB perception initiates various defence signalling events, such as activation of Ca2+ permeable channels, mitogen-activated protein kinases, and production of Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase-mediated reactive oxygen species. Furthermore, HB-mediated stomata closure was found to be independent of abscisic acid biosynthesis and signalling. Additionally, exogenous treatments with HB alleviate water stress and improve fruit productivity in tomato plants. The efficacy of HB was also tested under open field conditions, leading to enhanced resistance against Phytophthora spp. and Pseudomonas syringae infection in potato and tomato plants, respectively. Taken together, our results provide insights into the HB signalling transduction pathway, confirming its role in stomatal closure and plant immune system activation, and propose HB as a new phytoprotectant for the sustainable control of biotic and abiotic stresses in agriculture.