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Article|08 Nov 2023|OPEN
Berry texture QTL and candidate gene analysis in grape (Vitis vinifera L.) 
Hong Lin1 ,† , Li Ma1 ,† , Qiuyu Guo1 , Cheng Liu1 , Yangming Hou1 , Zhendong Liu1 , Yuhui Zhao1 , Changyue Jiang1 , Xiuwu Guo1 and Yinshan Guo,1,2 ,
1College of Horticulture, Shenyang Agricultural University, 120 Dongling Road, Shenyang, 110866, China
2Ministry of Education Key Laboratory of Protected Horticulture, Shenyang Agricultural University, Shenyang, 110866, China
*Corresponding author. E-mail: guoyinshan77@syau.edu.cn
Both authors contributed equally to the study.

Horticulture Research 11,
Article number: uhad226 (2024)
doi: https://doi.org/10.1093/hr/uhad226
Views: 46

Received: 14 Aug 2023
Accepted: 26 Oct 2023
Published online: 08 Nov 2023

Abstract

Berry texture is a noteworthy economic trait for grape; however, the genetic bases and the complex gene expression and regulatory mechanism for the diverse changes in berry texture are still poorly understood. In this study, the results suggest that it is difficult to obtain high-mesocarp firmness (MesF) and high-pericarp puncture hardness (PPH) grape cultivars with high pericarp brittleness (PerB). The high-density linkage map was constructed using whole-genome resequencing based on 151 F1 individuals originating from intraspecific hybridization between the firm-flesh cultivar ‘Red Globe’ and soft-flesh cultivar ‘Muscat Hamburg’. The total length of the consensus map was 1613.17 cM, with a mean genetic distance between adjacent bin markers of 0.59 cM. Twenty-seven quantitative trait loci (QTLs) for berry MesF, PPH, and PerB were identified in linkage groups (LGs) 1, 3, 4, 6, 8, 9, 10, 11, 14, 16, and 17, including twelve QTLs that were firstly detected in LGs 6, 11, and 14. Fourteen promising candidate genes were identified from the stable QTL regions in LGs 10, 11, 14, and 17. In particular, VvWARK2 and VvWARK8 refer to chromosome 17 and are two promising candidate genes for MesF and PPH, as the VvWARK8 gene may increase pectin residue binding with WARK for high berry firmness maintenance and the allele for VvWARK2 carrying the ‘CC’ and ‘GA’ genotypes at Chr17:1836764 and Chr17:1836770 may be associated with non-hard texture grape cultivars. In addition, real-time quantitative polymerase chain reaction (RT–qPCR) verification revealed that the promising candidate transcription factor genes VvMYB4-likeVvERF113VvWRKY31VvWRKY1, and VvNAC83 may regulate cell wall metabolism candidate gene expression for grape berry texture changes.