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Article|12 Apr 2023|OPEN
Pineapple SWEET10 is a glucose transporter
Beenish Fakher1,2 , M. Arif Ashraf3 , Lulu Wang1,2 , Xiaomei Wang4 and Ping Zheng2 , Mohammad Aslam2,5 , , Yuan Qin,2
1State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Key Lab of Sugarcane Biology, College of Agriculture, Guangxi University, Nanning 530004, Guangxi, China
2College of Life Sciences, Key Laboratory of Genetics, Breeding and Multiple Utilization of Crops, Ministry of Education, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China
3Department of Biology, Howard University, Washington DC 20059, USA
4Horticulture Research Institute, Guangxi Academy of Agricultural Sciences, Nanning Investigation Station of South Subtropical Fruit Trees, Ministry of Agriculture, Nanning 530004, China
5Donald Danforth Plant Science Center, Saint Louis, MO 63132, USA
*Corresponding author. E-mail: maslam@danforthcenter.org

Horticulture Research 10,
Article number: uhad175 (2023)
doi: https://doi.org/10.1093/hr/uhad175
Views: 34


Revised: 01 Sep 2023
Accepted: 25 Aug 2023
Published online: 12 Apr 2023

Abstract

SWEET transporters are a unique class of sugar transporters that play vital roles in various developmental and physiological processes in plants. While the functions of SWEETs have been well established in model plants such as Arabidopsis, their functions in economically important fruit crops like pineapple have not been well studied. Here we aimed to investigate the substrate specificity of pineapple SWEETs by comparing the protein sequences of known glucose and sucrose transporters in Arabidopsis with those in pineapple. Our genome-wide approach and 3D structure comparison showed that the Arabidopsis SWEET8 homolog in pineapple, AcSWEET10, shares similar sequences and protein properties responsible for glucose transport. To determine the functional conservation of AcSWEET10, we tested its ability to complement glucose transport mutants in yeast and analyzed its expression in stamens and impact on the microspore phenotype and seed set in transgenic Arabidopsis. The results showed that AcSWEET10 is functionally equivalent to AtSWEET8 and plays a critical role in regulating microspore formation through the regulation of the Callose synthase5 (CalS5), which highlights the importance of SWEET transporters in pineapple. This information could have important implications for improving fruit crop yield and quality by manipulating SWEET transporter activity.