Browse Articles

Article|29 Aug 2023|OPEN
VvWRKY5 enhances white rot resistance in grape by promoting the jasmonic acid pathway
Zhen Zhang1 , Changyue Jiang1 , Cui Chen1 , Kai Su2 , Hong Lin1 , , Yuhui Zhao1 and Yinshan Guo,1,3 ,
1College of Horticulture, Shenyang Agricultural University, 120 Dongling Road, Shenyang, Liaoning 110866, China
2College of Horticulture Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao 066004, China
3National & Local Joint Engineering Research Center of Northern Horticultural Facilities Design and Application Technology (Liaoning), Shenyang 110866, China
*Corresponding author. E-mail:,

Horticulture Research 10,
Article number: uhad172 (2023)
Views: 50

Received: 17 May 2023
Accepted: 20 Aug 2023
Published online: 29 Aug 2023


Grape white rot is a disease caused by Coniella diplodiella (Speg.) Sacc. (Cd) can drastically reduce the production and quality of grape (Vitis vinifera). WRKY transcription factors play a vital role in the regulation of plant resistance to pathogens, but their functions in grape white rot need to be further explored. Here, we found that the expression of the WRKY IIe subfamily member VvWRKY5 was highly induced by Cd infection and jasmonic acid (JA) treatment. Transient injection and stable overexpression (in grape calli and Arabidopsis) demonstrated that VvWRKY5 positively regulated grape resistance to white rot. We also determined that VvWRKY5 regulated the JA response by directly binding to the promoters of VvJAZ2 (a JA signaling suppressor) and VvMYC2 (a JA signaling activator), thereby inhibiting and activating the transcription of VvJAZ2 and VvMYC2, respectively. Furthermore, the interaction between VvJAZ2 and VvWRKY5 enhanced the suppression and promotion of VvJAZ2 and VvMYC2 activities by VvWRKY5, respectively. When VvWRKY5 was overexpressed in grape, JA content was also increased. Overall, our results suggested that VvWRKY5 played a key role in regulating JA biosynthesis and signal transduction as well as enhancing white rot resistance in grape. Our results also provide theoretical guidance for the development of elite grape cultivars with enhanced pathogen resistance.