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Article|16 Aug 2023|OPEN
An effector of Erysiphe necator translocates to chloroplasts and plasma membrane to suppress host immunity in grapevine 
Bo Mu1,2 , Zhaolin Teng1,2 , Ruixin Tang1,2 and Mengjiao Lu1,2 , Jinfu Chen1,2 , Xiangnan Xu3 , Ying-Qiang Wen,1,2 ,
1State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Yangling 712100, Shaanxi, China
2Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture and Rural Affairs, Yangling 712100, Shaanxi, China
3College of Water Resources and Architectural Engineering, Northwest A&F University, Weihui Road 23, Yangling 712100, Shaanxi, China
*Corresponding author. E-mail:

Horticulture Research 10,
Article number: uhad163 (2023)
Views: 119

Received: 24 Feb 2023
Accepted: 05 Aug 2023
Published online: 16 Aug 2023


The powdery mildew (Erysiphe necator) is a prevalent pathogen hampering grapevine growth in the vineyard. An arsenal of candidate secreted effector proteins (CSEPs) was encoded in the E. necator genome, but it is largely unclear what role CSEPs plays during the E. necator infection. In the present study, we identified a secreted effector CSEP080 of E. necator, which was located in plant chloroplasts and plasma membrane. Transient expressing CSEP080 promotes plant photosynthesis and inhibits INF1-induced cell death in tobacco leaves. We found that CSEP080 was a necessary effector for the E. necator pathogenicity, which interacted with grapevine chloroplast protein VviB6f (cytochrome b6-f complex iron–sulfur subunit), affecting plant photosynthesis. Transient silencing VviB6f increased the plant hydrogen peroxide production, and the plant resistance to powdery mildew. In addition, CSEP080 manipulated the VviPE (pectinesterase) to promote pectin degradation. Our results demonstrated the molecular mechanisms that an effector of E. necator translocates to host chloroplasts and plasma membrane, which suppresses with the grapevine immunity system by targeting the chloroplast protein VviB6f to suppress hydrogen peroxide accumulation and manipulating VviPE to promote pectin degradation.