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Article|11 Jul 2023|OPEN
CsBZIP40 confers resistance against citrus bacterial canker by repressing CsWRKY43-CsPrx53/CsSOD13 cascade mediated ROS scavenging
Qiang Li1,2,3 , , Xiujuan Qin1 , Miao Zhang1 , Qiyuan Yu1 , Ruirui Jia1 , Jie Fan1 , Xin Huang1 , Jia Fu1 , Chenxi Zhang1 , Wen Yang1 , Qin Long1,2,3 , Aihong Peng1,2,3 , Lixiao Yao1,2,3 , Shanchun Chen1,2,3 , , Yongrui He,1,2,3 ,
1Citrus Research Institute, Southwest University/Chinese Academy of Agricultural Sciences, Beibei, Chongqing 400712, China
2National Citrus Engineering Research Center, Beibei, Chongqing 400712, China
3National Citrus Improvement Center, Southwest University, Chongqing 400712, China
*Corresponding author. E-mail:,,

Horticulture Research 10,
Article number: uhad138 (2023)
Views: 107

Received: 20 Apr 2023
Accepted: 05 Jul 2023
Published online: 11 Jul 2023


As the bacterial etiologic agent causing citrus bacterial canker (CBC), Xanthomonas citri subsp. citri (Xcc) seriously impacts citrus plantation and fruit production globally. In an earlier study, we demonstrated that CsBZIP40 can positively impact CBC resistance in the sweet orange (Citrus sinensis). However, the mechanistic basis for the protective benefits conferred by CsBZIP40 is yet to be delineated. Here, we show that CsBZIP40 positively regulates CBC resistance and reactive oxygen species (ROS) homeostasis in transgenic sweet orange overexpressing CsBZIP40. CsBZIP40 directly binds to the TGA-box of the CsWRKY43 promoter to repress its transcriptional activity. CsWRKY43 overexpression induces CBC susceptibility in transgenic sweet oranges. In contrast, its inhibition produces strong resistance to CBC. CsWRKY43 directly binds to the W-boxes of the CsPrx53 and CsSOD13 promoters to positively regulate the activities of these antioxidant enzymes, resulting in the negative regulation of ROS homeostasis and CBC resistance in sweet orange plants. CsPrx53/CsSOD13 knockdown enhances ROS accumulation and CBC resistance. Overall, our results outline a regulatory pathway through which CsBZIP40 transcriptionally represses CsWRKY43-CsPrx53/CsSOD13 cascade-mediated ROS scavenging in a manner conducive to CBC resistance. These mechanisms underscore the potential importance of CsBZIP40, CsWRKY43, CsPrx53, and CsSOD13, providing promising strategies for the prevention of CBC.