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Article|31 May 2023|OPEN
VqMAPK3/VqMAPK6, VqWRKY33, and VqNSTS3 constitute a regulatory node in enhancing resistance to powdery mildew in grapevine
Wandi Liu1,2,3 ,† , Chaohui Yan1,2,3 ,† , Ruimin Li1,2,3 and Guanyu Chen1,2,3 , Xinqi Wang1,2,3 , Yingqiang Wen1,2,3 , Chaohong Zhang1,2,3 , Xiping Wang1,2,3 , Yan Xu1,2,3 , , Yuejin Wang,1,2,3 ,
1College of Horticulture, Northwest A & F University, Yangling, Shaanxi, 712100, China
2Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Yangling, Shaanxi, 712100, China
3State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A & F University, Yangling, Shaanxi, 712100, China
*Corresponding author. E-mail: yan.xu@nwsuaf.edu.cn,wangyj@nwsuaf.edu.cn
†Both authors contributed equally to the study.

Horticulture Research 10,
Article number: uhad116 (2023)
doi: https://doi.org/10.1093/hr/uhad116
Views: 193

Received: 30 Mar 2023
Accepted: 21 May 2023
Published online: 31 May 2023

Abstract

Grapevine powdery mildew is caused by Erysiphe necator, which seriously harms grape production in the world. Stilbene synthase makes phytoalexins that contribute to the resistance of grapevine against powdery mildew. A novel VqNSTS3 was identified and cloned from Chinese wild Vitis quinquangularis accession Danfeng-2. The novel VqNSTS3 was transferred into susceptible ‘Thompson Seedless’ by Agrobacterium-mediated transformation. The transgenic plants showed resistance to the disease and activated other resistance-related genes. VqNSTS3 expression in grapevine is regulated by VqWRKY33, and which binds to TTGACC in the VqNSTS3 promoter. Furthermore, VqWRKY33 was phosphorylated by VqMAPK3/VqMAPK6 and thus led to enhanced signal transduction and increased VqNSTS3 expression. ProVqNSTS3::VqNSTS3-GFP of transgenic VqNSTS3 in Arabidopsis thaliana was observed to move to and wrap the pathogen’s haustoria and block invasion by Golovinomyces cichoracearum. These results demonstrate that stilbene accumulation of novel VqNSTS3 of the Chinese wild Vitis quinquangularis accession Danfeng-2 prevented pathogen invasion and enhanced resistance to powdery mildew. Therefore, VqNSTS3 can be used in generating powdery mildew-resistant grapevines.