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Article|31 May 2023|OPEN
FvWRKY50 is an important gene that regulates both vegetative growth and reproductive growth in strawberry
Yating Chen1 , Liping Liu1 , Qianqian Feng1 , Chuang Liu1 , Yujuan Bao1 , Nan Zhang1 , Ronghui Sun1 , Zhaonan Yin1 , Chuanfei Zhong2 , Yuanhua Wang3,4 and Qian Li1 , , Bingbing Li,1 ,
1Department of Pomology, College of Horticulture, China Agricultural University, Beijing, 10093, China
2Beijing Engineering Research Center for Strawberry, Institute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100093, China
3Department of Agronomy and Horticulture, Jiangsu Vocational College of Agriculture and Forestry, Jiangsu, 212400, China
4Engineering and Technical Center for Modern Horticulture, Jiangsu, 212400, China
*Corresponding author. E-mail:,

Horticulture Research 10,
Article number: uhad115 (2023)
Views: 149

Received: 13 Apr 2023
Accepted: 21 May 2023
Published online: 31 May 2023


The WRKY transcription factors play important roles in plant growth and resistance, but only a few members have been identified in strawberry. Here we identified a WRKY transcription factor, FvWRKY50, in diploid strawberry which played essential roles in strawberry vegetative growth, and reproductive growth. Knocking out FvWRKY50 by genome editing accelerated flowering time and leaf senescence but delayed anthocyanin accumulation in fruit. Further analysis showed that FvWRKY50 acted as a transcriptional repressor to negatively regulate the expression of flowering- and leaf senescence-related genes, including FvFT2FvCOFvFT3, and FvSAUR36. Notably, FvWRKY50 directly upregulated the expression of FvCHI and FvDFR by binding their promoter under normal conditions, but at low temperature FvWRKY50 was phosphorylated by FvMAPK3 and then induced protein degradation by ubiquitination, delaying anthocyanin accumulation. In addition, the homozygous mutant of FvWRKY50 was smaller while the biallelic mutant showed normal size. These new findings provide important clues for us to further reveal the regulatory mechanisms of strawberry growth and fruit ripening