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Article|03 May 2023|OPEN
LncRNA109897-JrCCR4-JrTLP1b forms a positive feedback loop to regulate walnut resistance against anthracnose caused by Colletotrichum gloeosporioides 
Rui Zhou1 ,† , Yuhui Dong1 ,† , Changxi Wang1 , Jianning Liu1 , Qiang Liang1 , Xiaoye Meng2 , Xinya Lang1 , Shengyi Xu1 , Wenjun Liu3 , Shuhui Zhang3 , Nan Wang3 , Ke Qiang Yang1 , and Hongcheng Fang,1 ,
1State Forestry and Grassland Administration Key Laboratory of Silviculture in the Downstream Areas of the Yellow River, Shandong Taishan Forest Ecosystem Research Station, College of Forestry, Shandong Agricultural University, Tai’an, Shandong, China, 271018
2Department of Natural Resources Of Shandong Province, Forestry Protection and Development Service Center, Jinan, Shandong, China, 250000
3State Key Laboratory of Crop Biology, College of Horticulture Sciences and Engineering, Shandong Agricultural University, Tai’an, Shandong, China, 271018
*Corresponding author. E-mail: yangwere@126.com,cheng2011412hui@163.com
†Both authors contributed equally to the study.

Horticulture Research 10,
Article number: uhad086 (2023)
doi: https://doi.org/10.1093/hr/uhad086
Views: 255

Received: 11 Jan 2023
Accepted: 20 Apr 2023
Published online: 03 May 2023

Abstract

Walnut anthracnose induced by Colletotrichum gloeosporioides is a disastrous disease that severely restricts the development of the walnut industry in China. Long non-coding RNAs (lncRNAs) are involved in adaptive responses to disease, but their roles in the regulation of walnut anthracnose resistance response are not well defined. In this study, transcriptome analysis demonstrated that a C. gloeosporioides-induced lncRNA, lncRNA109897, located upstream from the target gene JrCCR4, upregulated the expression of JrCCR4. JrCCR4 interacted with JrTLP1b and promoted its transcriptional activity. In turn, JrTLP1b induced the transcription of lncRNA109897 to promote its expression. Meanwhile, transient expression in walnut leaves and stable transformation of Arabidopsis thaliana further proved that lncRNA, JrCCR4, and JrTLP1b improve the resistance of C. gloeosporioides. Collectively, these findings provide insights into the mechanism by which the lncRNA109897-JrCCR4-JrTLP1b transcriptional cascade regulates the resistance of walnut to anthracnose.