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Article|21 Apr 2023|OPEN
CRISPR/Cas9-mediated gene editing to confer turnip mosaic virus (TuMV) resistance in Chinese cabbage (Brassica rapa
Ye-Rin Lee1 , Muhammad Irfan Siddique2 , Do-Sun Kim1 , Eun Su Lee1 , Koeun Han1 , Sang-Gyu Kim3 and Hye-Eun Lee,1 ,
1Vegetable Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Wanju, 55365, Republic of Korea
2Department of Horticultural Sciences, North Carolina State University Mountain Horticultural Crops Research, Extension Center 455 Research Drive, Mills River, NC 28759, USA
3Department of Biological Sciences, Korea Advanced Institute for Science and Technology, Daejeon, 34141, Republic of Korea
*Corresponding author. E-mail:

Horticulture Research 10,
Article number: uhad078 (2023)
Views: 176

Received: 28 Nov 2022
Accepted: 10 Apr 2023
Published online: 21 Apr 2023


Genome editing approaches, particularly the CRISPR/Cas9 technology, are becoming state-of-the-art for trait development in numerous breeding programs. Significant advances in improving plant traits are enabled by this influential tool, especially for disease resistance, compared to traditional breeding. One of the potyviruses, the turnip mosaic virus (TuMV), is the most widespread and damaging virus that infects Brassica spp. worldwide. We generated the targeted mutation at the eIF(iso)4E gene in the TuMV-susceptible cultivar “Seoul” using CRISPR/Cas9 to develop TuMV-resistant Chinese cabbage. We detected several heritable indel mutations in the edited T0 plants and developed T1 through generational progression. It was indicated in the sequence analysis of the eIF(iso)4E-edited T1 plants that the mutations were transferred to succeeding generations. These edited T1 plants conferred resistance to TuMV. It was shown with ELISA analysis the lack of accumulation of viral particles. Furthermore, we found a strong negative correlation (r = −0.938) between TuMV resistance and the genome editing frequency of eIF(iso)4E. Consequently, it was revealed in this study that CRISPR/Cas9 technique can expedite the breeding process to improve traits in Chinese cabbage plants.