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Review Article|19 Apr 2023|OPEN
Raman spectroscopy in crop quality assessment: focusing on sensing secondary metabolites: a review
Miri Park1 , , Annette Somborn1 , Dennis Schlehuber1 , Volkmar Keuter1 and Görge Deerberg,1
1Fraunhofer Institute for Environmental, Safety and Energy Technologies UMSICHT, 46047, Oberhausen, Germany
*Corresponding author. E-mail:

Horticulture Research 10,
Article number: uhad074 (2023)
Views: 256

Received: 09 Dec 2022
Accepted: 12 Apr 2023
Published online: 19 Apr 2023


As a crop quality sensor, Raman spectroscopy has been consistently proposed as one of the most promising and non-destructive methods for qualitative and quantitative analysis of plant substances, because it can measure molecular structures in a short time without requiring pretreatment along with simple usage. The sensitivity of the Raman spectrum to target chemicals depends largely on the wavelength, intensity of the laser power, and exposure time. Especially for plant samples, it is very likely that the peak of the target material is covered by strong fluorescence effects. Therefore, methods using lasers with low energy causing less fluorescence, such as 785 nm or near-infrared, are vigorously discussed. Furthermore, advanced techniques for obtaining more sensitive and clear spectra, like surface-enhanced Raman spectroscopy, time-gated Raman spectroscopy or combination with thin-layer chromatography, are being investigated. Numerous interpretations of plant quality can be represented not only by the measurement conditions but also by the spectral analysis methods. Up to date, there have been attempted to optimize and generalize analysis methods. This review summarizes the state of the art of micro-Raman spectroscopy in crop quality assessment focusing on secondary metabolites, from in vitro to in vivo and even in situ, and suggests future research to achieve universal application.