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Article|10 Apr 2023|OPEN
A molecular toolkit to boost functional genomic studies in transformation-recalcitrant vegetable legumes
Xinyang Wu1 , Peipei Zhang2 , Shuting Chen1 , Zixin Zhang1 , Yihan Zhang1 , Pingping Fang1 , Kang Ning1 , Ting Sun1 and Pei Xu,1 ,
1Key Laboratory of Specialty Agri-Product Quality and Hazard Controlling Technology of Zhejiang, College of Life Sciences, China Jiliang University, Hangzhou, China
2Zhejiang Lab, Hangzhou, China
*Corresponding author. E-mail:

Horticulture Research 10,
Article number: uhad064 (2023)
Views: 182

Received: 31 Jan 2023
Accepted: 02 Apr 2023
Published online: 10 Apr 2023


Dear Editor

Legumes, the second-largest family of crops, contribute over one-third of human dietary proteins. Soybean (Glycine max L.), common bean (Phaseolus vulgaris L.), pea (Pisum sativum L.), and cowpea (Vigna unguiculata L.) are among the most widely cultivated crop legumes for grain and vegetable and are essential for food security globally. Their reference genomes have been decoded but, with the only exception of soybean, their functional genomics have lagged far behind genome assembly due to the transformation-recalcitrant nature of these species [1]. Hairy root transformation has been well established, but its usefulness in gene function investigation in aerial organs is limited. Virus-induced gene silencing (VIGS) has also found applications, but the most widely distributed bean pod mosaic virus-based system relies on the costly biolistic method [2]; other systems, like pea early browning virus-based VIGS, have not been proved for their universality. Genetic and omics studies have identified numerous quantitative trait loci (QTLs)/candidate genes governing various agriculturally important traits [1]. This necessitates the development of efficient and reproducible research tools for verification of gene function.