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Article|28 Mar 2023|OPEN
Discovery and genome-guided mapping of REN12 from Vitis amurensis, conferring strong, rapid resistance to grapevine powdery mildew
Surya Sapkota1 , Cheng Zou2 , Craig Ledbetter3 , Anna Underhill4 , Qi Sun2 , David Gadoury1 and Lance Cadle-Davidson,1,4 ,
1School of Integrative Plant Science, Cornell AgriTech, Cornell University, Geneva, NY, 14456, USA
2BRC Bioinformatics Facility, Institute of Biotechnology, Cornell University, Ithaca, NY, 14853, USA
3United States Department of Agriculture (USDA)-Agricultural Research Service (ARS), Crop Diseases, Pests and Genetics Research Unit, San Joaquin Valley Agricultural Sciences Center, Parlier, CA, 93648, USA
4USDA-ARS, Grape Genetics Research Unit, Geneva, NY, 14456, USA
*Corresponding author. E-mail:

Horticulture Research 10,
Article number: uhad052 (2023)
Views: 192

Received: 30 Sep 2022
Accepted: 12 Mar 2023
Published online: 28 Mar 2023


Powdery mildew resistance genes restrict infection attempts at different stages of pathogenesis. Here, a strong and rapid powdery mildew resistance phenotype was discovered from Vitis amurensis ‘PI 588631’ that rapidly stopped over 97% of Erysiphe necator conidia, before or immediately after emergence of a secondary hypha from appressoria. This resistance was effective across multiple years of vineyard evaluation on leaves, stems, rachises, and fruit and against a diverse array of E. necator laboratory isolates. Using core genome rhAmpSeq markers, resistance mapped to a single dominant locus (here named REN12) on chromosome 13 near 22.8–27.0 Mb, irrespective of tissue type, explaining up to 86.9% of the phenotypic variation observed on leaves. Shotgun sequencing of recombinant vines using skim-seq technology enabled the locus to be further resolved to a 780 kb region, from 25.15 to 25.93 Mb. RNASeq analysis indicated the allele-specific expression of four resistance genes (NLRs) from the resistant parent. REN12 is one of the strongest powdery mildew resistance loci in grapevine yet documented, and the rhAmpSeq sequences presented here can be directly used for marker-assisted selection or converted to other genotyping platforms. While no virulent isolates were identified among the genetically diverse isolates and wild populations of E. necator tested here, NLR loci like REN12 are often race-specific. Thus, stacking of multiple resistance genes and minimal use of fungicides should enhance the durability of resistance and could enable a 90% reduction in fungicides in low-rainfall climates where few other pathogens attack the foliage or fruit.