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Article|15 Mar 2023|OPEN
The first chromosome-level Fallopia multiflora genome assembly provides insights into stilbene biosynthesis 
Yujiao Zhao1,3 ,† , Zhengyang Yang1 ,† , Zhongren Zhang5 ,† , Minzhen Yin1 ,† , Shanshan Chu1,3 , Zhenzhen Tong1 , Yuejian Qin1 , Liangping Zha1,3 and Qingying Fang1,3 , Yuan Yuan2 , , Luqi Huang2 , , Huasheng Peng,1,2,4 ,
1School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China
2National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
3Anhui Province Key Laboratory of Research & Development of Chinese Medicine, Hefei 230012, China
4Research Unit of DAO-DI Herbs, Chinese Academy of Medical Sciences, 2019RU57, Beijing 100700, China
5Novogene Bioinformatics Institute, Beijing 301700, China
*Corresponding author. E-mail:,,
Yujiao Zhao,Zhengyang Yang and Zhongren Zhang,Minzhen Yin contributed equally to the study.

Horticulture Research 10,
Article number: uhad047 (2023)
Views: 174

Received: 30 Oct 2022
Accepted: 07 Mar 2023
Published online: 15 Mar 2023


Fallopia multiflora (Thunb.) Harald, a vine belonging to the Polygonaceae family, is used in traditional medicine. The stilbenes contained in it have significant pharmacological activities in anti-oxidation and anti-aging. This study describes the assembly of the F. multiflora genome and presents its chromosome-level genome sequence containing 1.46 gigabases of data (with a contig N50 of 1.97 megabases), 1.44 gigabases of which was assigned to 11 pseudochromosomes. Comparative genomics confirmed that F. multiflora shared a whole-genome duplication event with Tartary buckwheat and then underwent different transposon evolution after separation. Combining genomics, transcriptomics, and metabolomics data to map a network of associated genes and metabolites, we identified two FmRS genes responsible for the catalysis of one molecule of p-coumaroyl-CoA and three molecules of malonyl-CoA to resveratrol in F. multiflora. These findings not only serve as the basis for revealing the stilbene biosynthetic pathway but will also contribute to the development of tools for increasing the production of bioactive stilbenes through molecular breeding in plants or metabolic engineering in microbes. Moreover, the reference genome of F. multiflora is a useful addition to the genomes of the Polygonaceae family.