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Article|08 Feb 2023|OPEN
Development of homozygous tetraploid potato and whole genome doubling-induced the enrichment of H3K27ac and potentially enhanced resistance to cold-induced sweetening in tubers
Hongwei Guo1 ,† , Min Zhou1 ,† , Guoyan Zhang1,4 ,† , Li He4 ,† , Caihong Yan1 , Min Wan1 , Jianjun Hu3 , Wei He3 , Deying Zeng1,2 , Bo Zhu1,2 , , Zixian Zeng,1,2 ,
1Department of Biological Science, College of Life Sciences, Sichuan Normal University, Chengdu 610101, Sichuan, China
2Plant Functional Genomics and Bioinformatics Research Center, Sichuan Normal University, Chengdu 610101, Sichuan, China
3Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China
4Horticulture Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China
*Corresponding author. E-mail:,
Hongwei Guo,Min Zhou,Guoyan Zhang,Li He contributed equally to the study.

Horticulture Research 10,
Article number: uhad017 (2023)
Views: 368

Received: 27 Aug 2022
Accepted: 30 Jan 2023
Published online: 08 Feb 2023


Polyploid plants typically display advantages on some agronomically important traits over their diploid counterparts. Extensive studies have shown genetic, transcriptomic, and epigenetic dynamics upon polyploidization in multiple plant species. However, few studies have unveiled those alternations imposed only by ploidy level, without any interference from heterozygosity. Cultivated potato is highly heterozygous. Thus, in this study, we developed two homozygous autotetraploid lines and one homozygous diploid line in parallel from a homozygous diploid potato. We confirmed their ploidy levels using chloroplast counting and karyotyping. Oligo-FISH and genome re-sequencing validated that these potato lines are nearly homozygous. We investigated variations in phenotypes, transcription, and histone modifications between two ploidies. Both autotetraploid lines produced larger but fewer tubers than the diploid line. Interestingly, each autotetraploid line displayed ploidy-related differential expression for various genes. We also discovered a genome-wide enrichment of H3K27ac in genic regions upon whole-genome doubling (WGD). However, such enrichment was not associated with the differential gene expression between two ploidies. The tetraploid lines may exhibit better resistance to cold-induced sweetening (CIS) than the diploid line in tubers, potentially regulated through the expression of CIS-related key genes, which seems to be associated with the levels of H3K4me3 in cold-stored tubers. These findings will help to understand the impacts of autotetraploidization on dynamics of phenotypes, transcription, and histone modifications, as well as on CIS-related genes in response to cold storage.