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Article|11 Jan 2023|OPEN
Jasmonic acid regulates the biosynthesis of medicinal metabolites via the JAZ9-MYB76 complex in Salvia miltiorrhiza 
Shucan Liu1,2 ,† , Xiankui Gao1 ,† , Min Shi1 , Meihong Sun3 , Kunlun Li1 , Yan Cai1 , Chengan Chen1 , Can Wang1 , Itay Maoz4 and Xinhong Guo2 , , Guoyin Kai,1 ,
1Laboratory of Medicinal Plant Biotechnology, School of Pharmaceutical Sciences, Jinhua Academy, Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, China
2College of Biology, Hunan University, Changsha, Hunan 410082, China
3Institute of Plant Biotechnology, School of Life Sciences, Shanghai Normal University, Shanghai 200234, China
4Department of Postharvest Science, ARO, The Volcani Center, HaMaccabim Rd 68, POB 15159, Rishon LeZion, 7528809, Israel
*Corresponding author. E-mail:,
Both authors contributed equally to the study.

Horticulture Research 10,
Article number: uhad004 (2023)
Views: 220

Received: 03 Nov 2022
Accepted: 03 Jan 2023
Published online: 11 Jan 2023


Jasmonic acid (JA) signaling pathway plays an important role in tanshinone and phenolic acid biosynthesis in Salvia miltiorrhiza. However, the specific regulatory mechanism remains largely unclear. Previous work showed that a JASMONATE ZIM-domain (JAZ) protein, SmJAZ9, acted as a repressor of tanshinone production in S. miltiorrhiza. In this study, we revealed that SmJAZ9 reduced both phenolic acid accumulation and related biosynthetic gene expression, confirming that SmJAZ9 also negatively affected phenolic acid biosynthesis. Then, we identified a novel MYB transcription factor, SmMYB76, which interacted with SmJAZ9. SmMYB76 repressed phenolic acid biosynthesis by directly downregulating SmPAL1Sm4CL2, and SmRAS1. Further investigation demonstrated that JA mediated phenolic acids biosynthesis via SmJAZ9-SmMYB76 complex. Taken together, these findings state the molecular mechanism that SmJAZ9-SmMYB76 regulated phenolic acid biosynthesis at the transcriptional and protein levels, which provided new insights into JA signaling pathway regulating plant metabolism.