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Article|23 Dec 2022|OPEN
H2S-mediated balance regulation of stomatal and non-stomatal factors responding to drought stress in Chinese cabbage 
Wenze Zhang1 ,† , Lei Wang1,2 ,† , Liping Zhang1 , Xiangqun Kong1 , Jiao Zhang1 , Xin Wang1 and Yanxi Pei1 , , Zhuping Jin,1 ,
1School of Life Science and Shanxi Key Laboratory for Research and Development of Regional Plants, Shanxi University, Taiyuan, Shanxi Province 030032, China
2Taiyuan Yuying High School, Taiyuan, Shanxi Province 030009, China
*Corresponding author. E-mail:,
Both authors contributed equally to the study.

Horticulture Research 10,
Article number: uhac284 (2023)
Views: 242

Received: 10 Aug 2022
Accepted: 08 Dec 2022
Published online: 23 Dec 2022


Increased evidence has shown that hydrogen sulfide (H2S), a novel gasotransmitter, could enhance drought resistance in plants by inducing stomatal closure, with concurrent enhancement of photosynthetic efficiency, but little is known about the mechanism behind this contradictory phenomenon. This study examined the regulating mechanism of H2S in response to drought stress from stomatal and non-stomatal factors in Chinese cabbage. The results showed that exogenous H2S could increase the accumulation of photosynthetic pigments and alleviate the damage caused by drought stress. It also regulated the expression in transcriptional level and the activity of ribulose 1,5-bisphosphate carboxylase/oxygenase (BrRuBisCO) under drought stress. The large subunit of BrRuBisCO was found to be modified by S-sulfhydration, which might be the reason for its increased enzyme activity. The fluxes of Cl, K+, and H+ in the guard cells were detected by non-invasive micro-test techniques while under drought stress. The results indicated that H2S signaling induced a transmembrane Cl and H+ efflux and inhibited K+ influx, and the Cl channel was the main responders for H2S-regulated stomatal movement. In conclusion, H2S signal not only activated the ion channel proteins located in the guard cell membrane to induce stomatal closure, but also regulated the transcriptional expression and the activity of RuBisCO, a non-stomatal factor to enhance the photosynthetic efficiency of leaves. There is therefore a beneficial balance between the regulation of H2S signaling on stomatal factors and non-stomatal factors due to drought stress, which needs to be better understood to apply it practically to increase crop yields.