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Article|22 Dec 2022|OPEN
SlMYB1 regulates the accumulation of lycopene, fruit shape, and resistance to Botrytis cinerea in tomato
Ziyi Yin1 ,† , Jiazong Liu1 ,† , Haipeng Zhao1 ,† , Xiaomeng Chu1 , Haoqi Liu1 , Xiangyu Ding1 , Chongchong Lu1 , Xinyu Wang1 , Xiangyu Zhao2 , Yang Li1,2 , , Xinhua Ding,1 ,
1State Key Laboratory of Crop Biology, Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of plant protection, Shandong Agricultural University, Taian 271018, Shandong, China
2State Key Laboratory of Crop Biology, College of Academy of Life Science, Shandong Agricultural University, Taian 271018, Shandong, China
*Corresponding author. E-mail: yangli1988@sdau.edu.cn,xhding@sdau.edu.cn
Ziyi Yin,Jiazong Liu and Haipeng Zhao contributed equally to the study.

Horticulture Research 10,
Article number: uhac282 (2023)
doi: https://doi.org/10.1093/hr/uhac282
Views: 282

Received: 10 Aug 2022
Accepted: 07 Dec 2022
Published online: 22 Dec 2022

Abstract

Fruit lycopene, shape, and resistance are essential traits in vegetables whose final product is fruit, and they are also closely related to and strictly regulated by multiple transcription factors. Lycopene, which cannot be synthesized by the human body and can only be ingested from the outside, was important in maintaining human health. During fruit ripening and post-harvest, tomato plants face a variety of biotic or abiotic stresses, which might inflict great damage to fruit quality due to its flat shape and pointed tip during storage and transportation. Therefore, there is an urgent need for key molecular switches to simultaneously improve fruit lycopene and resistance to biotic stress during ripening. Here, we identified the MYB transcription factor SlMYB1 in tomato plants which could bind to the promoters of lycopene synthesis-related genes, SlLCY1, SlPSY2, and the pathogen-related gene SlPR5 directly, to regulate the fruit lycopene and resistance to Botrytis cinerea in tomato. In addition to regulating lycopene synthesis, SlMYB1 also regulates the content of soluble sugar, soluble protein and flavonoid in tomato. What’s more, SlMYB1 could regulate the tomato fruit shape, making it smoother or flatter to prevent skin damage caused by vibration on fruits. RNA sequencing (RNA-seq) further showed that SlMYB1 fruit-specific expression lines had multiple differentially expressed genes compared with those from wild-type plants, suggesting that SlMYB1 might have multiple roles in fruit nutritional quality control and resistance to stresses, which is a rare occurrence in previous studies. In summary, our results revealed that SlMYB1 was an essential multi-functional transcription factor that could regulate the lycopene and resistance to Botrytis cinerea, and change the shape of fruit in tomato plants.