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Article|07 Dec 2022|OPEN
MaDREB1F confers cold and drought stress resistance through common regulation of hormone synthesis and protectant metabolite contents in banana
Yi Xu1,2,3 ,† , Wei Hu1,2,3,4 , ,† , Shun Song1,3 ,† , Xiaoxue Ye1,2,3,4 , Zehong Ding1,2,3,4 , Juhua Liu1,2,3,4 , Zhuo Wang1,2,3,4 , Jingyang Li1 , Xiaowan Hou5 , Biyu Xu1,2,4 , , Zhiqiang Jin,1,2,3,4 ,
1Haikou Experimental Station, Key Laboratory of Genetic Improvement of Bananas, Sanya Research Institute, State Key Laboratory of Biological Breeding for Tropical Crops, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Hainan, China
2Hainan Key Laboratory for Protection and Utilization of Tropical Bioresources, Hainan Institute for Tropical Agricultural Resources, Chinese Academy of Tropical Agricultural Sciences, Hainan, China
3Hainan Yazhou Bay Seed Laboratory, Hainan, China
4Hainan Key Laboratory for Biosafety Monitoring and Molecular Breeding in Off-Season Reproduction Regions, Key Laboratory of Biology and Genetic Resources of Tropical Crops, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Hainan, China
5Key Laboratory of Hainan Province for Postharvest Physiology and Technology of Tropical Horticultural Products, South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences, Guangdong, China
*Corresponding author. E-mail: huwei2010916@126.com,biyuxu@126.com,18689846976@163.com
Yi Xu,Wei Hu,Shun Song contributed equally to the study.

Horticulture Research 10,
Article number: uhac275 (2023)
doi: https://doi.org/10.1093/hr/uhac275
Views: 306

Received: 03 Sep 2022
Accepted: 02 Dec 2022
Published online: 07 Dec 2022

Abstract

Adverse environmental factors severely affect crop productivity. Improving crop resistance to multiple stressors is an important breeding goal. Although CBFs/DREB1s extensively participate in plant resistance to abiotic stress, the common mechanism underlying CBFs/DREB1s that mediate resistance to multiple stressors remains unclear. Here, we show the common mechanism for MaDREB1F conferring cold and drought stress resistance in banana. MaDREB1F encodes a dehydration-responsive element binding protein (DREB) transcription factor with nuclear localization and transcriptional activity. MaDREB1F expression is significantly induced after cold, osmotic, and salt treatments. MaDREB1F overexpression increases banana resistance to cold and drought stress by common modulation of the protectant metabolite levels of soluble sugar and proline, activating the antioxidant system, and promoting jasmonate and ethylene syntheses. Transcriptomic analysis shows that MaDREB1F activates or alleviates the repression of jasmonate and ethylene biosynthetic genes under cold and drought conditions. Moreover, MaDREB1F directly activates the promoter activities of MaAOC4 and MaACO20 for jasmonate and ethylene syntheses, respectively, under cold and drought conditions. MaDREB1F also targets the MaERF11 promoter to activate MaACO20 expression for ethylene synthesis under drought stress. Together, our findings offer new insight into the common mechanism underlying CBF/DREB1-mediated cold and drought stress resistance, which has substantial implications for engineering cold- and drought-tolerant crops.