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Article|02 Dec 2022|OPEN
Mapping and validation of the epistatic D and P genes controlling anthocyanin biosynthesis in the peel of eggplant (Solanum melongena L.) fruit
Qian You1 ,† , Huimin Li1,4 ,† , Jun Wu1,2 ,† , Tao Li1 , Yikui Wang3 and Guangwen Sun2 , , Zhiliang Li1 , , Baojuan Sun,1 ,
1Guangdong Key Laboratory for New Technology Research of Vegetables, Vegetable Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, Guangdong 510640, China
2College of Horticulture, South China Agricultural University, Guangzhou, Guangdong 510642, China
3Institute of Vegetable, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi 530007, China
4Ganzhou Research Institute of Vegetables and Flowers, Gannan Academy of Sciences, Ganzhou, Jiangxi 341400, China
*Corresponding author. E-mail: sungw1968@scau.edu.cn,vri_li@163.com,sunbaojuan@hotmail.com
Qian You,Huimin Li,Jun Wu contributed equally to the study.

Horticulture Research 10,
Article number: uhac268 (2023)
doi: https://doi.org/10.1093/hr/uhac268
Views: 260

Received: 06 Jul 2022
Accepted: 27 Nov 2022
Published online: 02 Dec 2022

Abstract

Fruit color is an important trait influencing the commercial value of eggplant fruits. Three dominant genes (DP and Y) cooperatively control the anthocyanin coloration in eggplant fruits, but none has been mapped. In this study, two white-fruit accessions (19 141 and 19 147) and their F2 progeny, with 9:7 segregation ratio of anthocyanin pigmented versus non-pigmented fruits, were used for mapping the D and P genes. A high-density genetic map was constructed with 5270 SNPs spanning 1997.98 cM. Three QTLs were identified, including two genes on chromosome 8 and one on chromosome 10. Gene expression analyses suggested that the SmANS on chromosome 8 and SmMYB1 on chromosome 10 were the putative candidate genes for P and D, respectively. We further identified (1) a SNP leading to a premature stop codon within the conserved PLN03176 domain of SmANS in 19 141, (2) a G base InDel in the promoter region leading to an additional cis-regulatory element and (3) a 6-bp InDel within the R2-MYB DNA binding domain of SmMYB1, in 19 147. Subsequently, these three variations were validated by PARMS technology as related to phenotypes in the F2 population. Moreover, silencing of SmANS or SmMYB1 in the purple red fruits of F1 (E3316) led to inhibition of anthocyanin biosynthesis in the peels. Conversely, overexpression of SmANS or SmMYB1 restored anthocyanin biosynthesis in the calli of 19 141 and 19 147 respectively. Our findings demonstrated the epistatic interactions underlying the white color of eggplant fruits, which can be potentially applied to breeding of eggplant fruit peel color.