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Article|09 Dec 2022|OPEN
Variations of stomata development in tea plant (Camellia sinensis) leaves in different light and temperature environments and genetic backgrounds
Ping Li1 , Junming Lin2 , Mingzhi Zhu2 , Hao Zuo1 , Yihua Shen1 , Juan Li2 , Kunbo Wang2 , Penghui Li1 , Qian Tang3 , Zhonghua Liu2 and Jian Zhao,1,2 ,
1State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei 230036, China
2Key Laboratory of Tea Science of Ministry of Education, College of Horticulture, Hunan Agricultural University, Changsha 410128, China
3College of Horticulture, Sichuan Agricultural University, Chengdu 611130, China
*Corresponding author. E-mail: zhaojian@ hunau.edu.cn

Horticulture Research 10,
Article number: uhac278 (2023)
doi: https://doi.org/10.1093/hr/uhac278
Views: 288

Received: 26 Aug 2022
Accepted: 01 Dec 2022
Published online: 09 Dec 2022

Abstract

Stomata perform important functions in plant photosynthesis, respiration, gas exchange, and interactions with environments. However, tea plant stomata development and functions are not known. Here, we show morphological changes during stomata development and genetic dissection of stomata lineage genes regulating stomata formation in tea developing leaves. Different tea plant cultivars displayed clear variations in the stomata development rate, density and size, which are closely related to their tolerance against dehydration capabilities. Whole sets of stomata lineage genes were identified to display predicted functions in regulating stomatal development and formation. The stomata development and lineage genes were tightly regulated by light intensities and high or low temperature stresses, which affected stomata density and function. Furthermore, lower stomatal density and larger size were observed in triploid tea varieties as compared to those in diploid plant. Key stomata lineage genes such as CsSPCHs, CsSCRM, and CsFAMA showed much lower expression levels, whereas negative regulators CsEPF1 and CsYODAs had higher expression levels in triploid than in diploid tea varieties. Our study provides new insight into tea plant stomatal morphological development and the genetic regulatory mechanisms on stomata development under abiotic stresses and genetic backgrounds. The study lays a foundation for future exploring of the genetic improvement of water use efficiency in tea plants for living up to the challenge of global climate change.