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Article|05 Sep 2022|OPEN
SlWRKY45 interacts with jasmonate-ZIM domain proteins to negatively regulate defense against the root-knot nematode Meloidogyne incognita in tomato
Huang Huang1,2 , ,† , Wenchao Zhao1,2 ,† , Hui Qiao1 ,† , Chonghua Li1 , Lulu Sun1,2 , Rui Yang2 and Xuechun Ma1 , Jilin Ma1 , Susheng Song3 , , Shaohui Wang,1,2 ,
1Plant Science and Technology College, Beijing University of Agriculture, Beijing, 102206, China
2Beijing Key Laboratory for Agricultural Application and New Technique, Beijing University of Agriculture, Beijing, 102206, China
3College of Life Sciences, Capital Normal University, Beijing, 100048, China
*Corresponding author. E-mail: hhuang2017bua@163.com,songsslab@163.com,wangshaohui@bua.edu.cn
Huang Huang and Wenchao Zhao,Hui Qiao contributed equally to the study.

Horticulture Research 9,
Article number: uhac197 (2022)
doi: https://doi.org/10.1093/hr/uhac197
Views: 98

Received: 12 May 2022
Accepted: 28 Aug 2022
Published online: 05 Sep 2022

Abstract

Parasitic root-knot nematodes (RKNs) cause a severe reduction in crop yield and seriously threaten agricultural production. The phytohormones jasmonates (JAs) are important signals regulating resistance to multiple biotic and abiotic stresses. However, the molecular mechanism for JAs-regulated defense against RKNs in tomato remains largely unclear. In this study, we found that the transcription factor SlWRKY45 interacted with most JA-ZIM domain family proteins (JAZs), key repressors of the JA signaling. After infection by the RKN Meloidogyne incognita, the slwrky45 mutants exhibited lower gall numbers and egg numbers per gram of roots than wild type, whereas overexpression of SlWRKY45 attenuated resistance to Meloidogyne incognita. Under M. incognita infection, the contents of jasmonic acid (JA) and JA-isoleucine (JA-Ile) in roots were repressed by SlWRKY45-overexpression. Furthermore, SlWRKY45 bound to and inhibited the promoter of the JA biosynthesis gene ALLENE OXIDE CYCLASE (AOC), and repressed its expression. Overall, our findings revealed that the SlJAZ-interaction protein SlWRKY45 attenuated RKN-regulated JA biosynthesis and repressed defense against the RKN M. incognita in tomato.