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Article|24 Aug 2022|OPEN
TcMYC2 regulates Pyrethrin biosynthesis in Tanacetum cinerariifolium 
Tuo Zeng1,2 ,† , Jia-Wen Li1 ,† , Zhi-Zhuo Xu1 , Li Zhou1 , Jin-Jin Li1 , Qin Yu1 , Jin Luo1 , Zhu-Long Chan1 , Maarten A. Jongsma3 and Hao Hu1,4 , , Cai-Yun Wang,1 ,
1Key Laboratory for Biology of Horticultural Plants, Ministry of Education, College of Horticulture & Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China
2School of Life Sciences, Guizhou Normal University, Guiyang 550000, China
3Business Unit Bioscience, Wageningen University and Research, Droevendaalsesteeg 1, 6708, PB Wageningen, the Netherlands
4Department of Botany and Plant Sciences, Institute of Integrative Genome Biology, University of California, Riverside, CA 92521, USA
*Corresponding author. E-mail: haohu@mail.hzau.edu.cn,wangcy@mail.hzau.edu.cn
Both authors contributed equally to the study.

Horticulture Research 9,
Article number: uhac178 (2022)
doi: https://doi.org/10.1093/hr/uhac178
Views: 86

Received: 10 May 2022
Accepted: 02 Aug 2022
Published online: 24 Aug 2022

Abstract

Pyrethrins constitute a class of terpene derivatives with high insecticidal activity and are mainly synthesized in the capitula of the horticulturally important plant, Tanacetum cinerariifolium. Treatment of T. cinerariifolium with methyl jasmonate (MeJA) in the field induces pyrethrin biosynthesis, but the mechanism linking MeJA with pyrethrin biosynthesis remains unclear. In this study, we explored the transcription factors involved in regulating MeJA-induced pyrethrin biosynthesis. A single spray application of MeJA to T. cinerariifolium leaves rapidly upregulated the expression of most known pyrethrin biosynthesis genes and subsequently increased the total pyrethrin content in the leaf. A continuous 2-week MeJA treatment resulted in enhanced pyrethrin content and increased trichome density. TcMYC2, a key gene in jasmonate signaling, was screened at the transcriptome after MeJA treatment. TcMYC2 positively regulated expression of the pyrethrin biosynthesis genes TcCHSTcAOC, and TcGLIP by directly binding to E-box/G-box motifs in the promoters. The stable overexpression of TcMYC2 in T. cinerariifolium hairy roots significantly increased the expression of TcAOC and TcGLIP. Further transient overexpression and viral-induced gene-silencing experiments demonstrated that TcMYC2 positively promoted pyrethrin biosynthesis. Collectively, the results reveal a novel molecular mechanism for MeJA-induced pyrethrin biosynthesis in T. cinerariifolium involving TcMYC2.