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Article|01 Aug 2022|OPEN
CRISPR/Cas9-mediated precise targeted mutagenesis of phytoene desaturase in celery
Jie-Xia Liu1 ,† , Tong Li1 ,† , Hao Wang1 , Yan-Hua Liu1 , Kai Feng1 , Ao-Qi Duan1 , Hui Liu1 , Sheng Shu1 and Ai-Sheng Xiong,1 ,
1State Key Laboratory of Crop Genetics and Germplasm Enhancement, Ministry of Agriculture and Rural Affairs Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in East China, College of Horticulture, Nanjing Agricultural University, 1 Weigang, Nanjing, 210095, China
*Corresponding author. E-mail: xiongaisheng@njau.edu.cn
Both authors contributed equally to the study.

Horticulture Research 9,
Article number: uhac162 (2022)
doi: https://doi.org/10.1093/hr/uhac162
Views: 85

Received: 25 Mar 2022
Accepted: 12 Jul 2022
Published online: 01 Aug 2022

Abstract

Dear Editor

Celery (Apium graveolens L.) is a leafy vegetable crop of Apiaceae with economic importance, which is widely cultivated all over the world [1]. In production, improving quality, disease, and insect resistance, and late bolting are required for celery through traditional or modern molecular genetic improvement methods. Genetic improvement via conventional breeding was limited to the long breeding cycle and randomness; the necessity of genetic engineering breeding, therefore, has been highlighted. A precise genome-editing technology holds the potential to overcome the limitations of conventional breeding. Additionally, the research of functional genomics in celery also raised higher requirements for the development of genome-editing technology. An immature genetic transformation system and undeveloped gene-editing technology has become the bottleneck for basic research and genetic improvement in celery relative to other major crops.