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Horticulture Research 9,
Article number: uhac161 (2022)
doi: https://doi.org/10.1093/hr/uhac161
Views: 322
Received: 20 May 2022
Accepted: 08 Jul 2022
Published online: 01 Aug 2022
Dear Editor,
For any species, a high-quality reference genome is the basis for almost all kinds of genomic analysis [1]. However, for decades the reference sequences of important eukaryotic genomes were incomplete due to the missing repetitive genomic regions including both tandem repeats such as centromere, telomere, and ribosomal DNA, and interspersed repeats like transposons and segmental duplications. The incomplete reference genomes not only cause data analytical mistakes like false-positive variant calls but also impede the studies of repeat-related diseases such as cancer and infertility [2, 3]. Fortunately, the generation of Pacbio high-fidelity (HiFi) long sequences and Oxford Nanopore Technology (ONT) ultra-long (UL) sequences provides the opportunity of solving repeat assembly problems because of their advantages in accuracy and length, respectively, and a list of complete (T2T, or Telomere to Telomere) or near-complete reference genomes of important eukaryotic species like human [4], Arabidopsis [5], rice [6] and tomato [7] have been built recently.