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Article|02 Jul 2022|OPEN
The fruit glossiness locus, dull fruit (D), encodes a C2H2-type zinc finger transcription factor, CsDULL, in cucumber (Cucumis sativus L.)
Xuling Zhai1,2 , Haoying Wu1,2 , Yaru Wang1,2 , Zhongren Zhang1,2 , Li Shan1,2 , Xi Zhao1,2 , Ruijia Wang1,2 , Chang Liu1,2 , Yiqun Weng3 , Ying Wang4 , Xingwang Liu1,2 , , Huazhong Ren,1,2 ,
1Engineering Research Center of the Ministry of Education for Horticultural Crops Breeding and Propagation, College of Horticulture, China Agricultural University, Beijing 100193, China
2Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, College of Horticulture, China Agricultural University, Beijing 100193, China
3USDA-ARS, Vegetable Crops Research Unit, Horticulture Department, University of Wisconsin, 1575 Linden Dr., Madison, WI 53706, USA
4Heze Agricultural and Rural Bureau, 1021 Shuanghe Road, Mudan District, Heze, Shandong, 274000, China
*Corresponding author. E-mail: liuxw01@cau.edu.cn,renhuazhong@cau.edu.cn

Horticulture Research 9,
Article number: uhac146 (2022)
doi: https://doi.org/10.1093/hr/uhac146
Views: 152

Received: 19 Feb 2022
Accepted: 22 Jun 2022
Published online: 02 Jul 2022

Abstract

Fruit glossiness is an important external fruit quality trait for fresh-consumed cucumber fruit, affecting its marketability. Dull fruit appearance is mainly controlled by a single gene, D (for dull fruit) that is dominant to glossy fruit (dd), but the molecular mechanism controlling fruit glossiness is unknown. In the present study, we conducted map-based cloning of the D locus in cucumber and identified a candidate gene (Csa5G577350) that encodes a C2H2-type zinc finger transcription factor, CsDULL. A 4895-bp deletion including the complete loss of CsDULL resulted in glossy fruit. CsDULL is highly expressed in the peel of cucumber fruit, and its expression level is positively correlated with the accumulation of cutin and wax in the peel. Through transcriptome analysis, yeast one-hybrid and dual-luciferase assays, we identified two genes potentially targeted by CsDULL for regulation of cutin and wax biosynthesis/transportation that included CsGPAT4 and CsLTPG1. The possibility that CsDULL controls both fruit glossiness and wart development in cucumber is discussed. The present work advances our understanding of regulatory mechanisms of fruit epidermal traits, and provides a useful tool for molecular breeding to improve external fruit quality in cucumber.