1CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Innovative Academy of Seed Design, Chinese Academy of Sciences, Wuhan 430074, China 2Hubei Hongshan Laboratory, Wuhan 430070, China 3University of Chinese Academy of Sciences, Beijing 100049, China 4Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, Zhejiang University, Hangzhou 310058, China 5Boyce Thompson Institute, Cornell University, Ithaca, NY 14853, USA 6US Department of Agriculture-Agricultural Research Service, Robert W. Holley Center for Agriculture and Health, Ithaca, NY 14853, USA *Corresponding author. E-mail: firstname.lastname@example.org,email@example.com,firstname.lastname@example.org †Both authors contributed equally to the study.
Received: 29 Mar 2022 Accepted: 12 Jun 2022 Published online: 20 Jun 2022
Introducing beneficial genes/alleles from wild relatives into the cultivated tomato has been an important approach for tomato breeding. Solanum habrochaites and S. galapagense have been widely used as germplasm donors in modern breeding to improve biotic and abiotic stress tolerance of tomato. S. habrochaites grows in the Peruvian Andes at altitudes up to 3300 m and is notable for its tolerance of chilling and drought and resistance to many diseases and pests. S. galapagense is endemic to the Galápagos Islands, has extraordinary salt tolerance and insect resistance, and appears even more closely related to the cultivated tomato (Solanum lycopersicum) than Solanum pimpinellifolium, the wild progenitor of cultivated tomato . Due to their importance, draft genomes of these two species have been assembled using Illumina short-read sequencing  or PacBio long-read sequencing . However, high levels of fragmentation and/or the lack of chromosome-scale assemblies have limited their applications in tomato breeding and research.