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Article|23 Mar 2022|OPEN
Construction of a collection of introgression lines of “Texas” almond DNA fragments in the “Earlygold” peach genetic background
Naveen Kalluri1 , Octávio Serra2 , José Manuel Donoso3 , Roger Picañol4 , Werner Howad1,5 and Iban Eduardo1,5 , , Pere Arús,1,5 ,
1Centre for Research in Agricultural Genomics (CRAG) CSIC-IRTA-UAB-UB, Campus UAB, Edifici CRAG, Cerdanyola del Vallès (Bellaterra), 08193 Barcelona, Spain
2Instituto Nacional de Investigação Agrária e Veterinária, I.P., Banco Português de Germoplasma Vegetal (BPGV), Braga, Portugal
3Instituto de Investigaciones Agropecuarias (INIA), Centro Regional de Investigación Rayentué, Av. Salamanca s/n Sector Los Choapinos, Rengo 2940000, Chile
4Rijk Zwaan Ibérica S.A. Finca La Marina-PJ Lo Contreras 30395, La Puebla|Cartagena (Murcia), Spain
5IRTA, Campus UAB, Edifici CRAG, Cerdanyola del Vallès (Bellaterra), 08193 Barcelona, Spain
*Corresponding author. E-mail: iban.eduardo@irta.cat,pere.arus@irta.cat

Horticulture Research 9,
Article number: uhac070 (2022)
doi: https://doi.org/10.1093/hr/uhac070
Views: 182

Received: 01 Dec 2021
Accepted: 01 Dec 2021
Published online: 23 Mar 2022

Abstract

Peach [Prunus persica L. Batsch] is one of the major temperate fruit tree species, the commercial materials of which have a low level of genetic variability. Almond [P. dulcis (Mill) DA Webb], a close relative of peach cultivated for its kernels, has a much higher level of diversity. The species are inter-compatible and often produce fertile hybrids, almond being a possible source of new genes for peach that could provide biotic and abiotic stress tolerance traits. In this paper we describe the development of a collection of peach-almond introgression lines (ILs) having a single fragment of almond (cv. Texas) in the peach background (cv. Earlygold). Lines with few introgressions were selected with markers from successive generations from a “Texas” × “Earlygold” F1 hybrid, initially using a set of SSRs and later with the 18 k peach SNP chip, allowing for the final extraction of 67 lines, 39 with almond heterozygous introgressions covering 99% of the genome, and 28 with homozygous introgressions covering 83% of the genome. As a proof of concept, four major genes and four quantitative characters were examined in the selected ILs giving results generally consistent with previous information on the genetics of these characters. This collection is the first of its kind produced in a woody perennial species and promises to be a valuable tool for genetic analyses, including dissection of quantitative traits, positional cloning, epistasis and as prebreeding material to introgress almond genes of interest into the peach commercial gene pool.