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Article|14 Mar 2022|OPEN
Sugar signal mediates flavonoid biosynthesis in tea leaves
Yi-Qing Lv1 , Da Li2 , Liang-Yu Wu3 , Yu-Meng Zhu4 , Ying Ye1 , Xin-Qiang Zheng1 , Jian-Liang Lu1 , Yue-Rong Liang1 , Qing-Sheng Li2 , and Jian-Hui Ye,1 ,
1Tea Research Institute, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, China
2Institute of Sericulture and Tea, Zhejiang Academy of Agricultural Sciences, 298 Deshengzhong Road, Hangzhou 310021, China
3College of Horticulture, Fujian Agriculture and Forestry University, 15 Shangxiadian Road, Fuzhou 350000, China
4Ningbo Yinzhou District Agricultural Technical Extension Station, 55 Huifengxi Road, Ningbo 315100, China
*Corresponding author. E-mail: liqs@zaas.ac.cn,jianhuiye@zju.edu.cn

Horticulture Research 9,
Article number: uhac049 (2022)
doi: https://doi.org/10.1093/hr/uhac049
Views: 22

Received: 03 Jan 2022
Accepted: 11 Feb 2022
Published online: 14 Mar 2022

Abstract

Sugar metabolism and flavonoid biosynthesis vary with the development of tea leaves. In order to understand the regulatory mechanisms underlying the associations between them, a comprehensive transcriptomic analysis of naturally growing tea leaves at different stages of maturity was carried out. Based on weighted gene coexpression network analysis, the key gene modules (Modules 2 and 3) related to the varying relationship between sugar metabolism and flavonoid biosynthesis as well as the corresponding hub genes were obtained. KEGG (Kyoto Encyclopedia of Genes and Genomes) enrichment analysis showed that the transcription factors (TFs) in Modules 2 and 3 were mainly enriched in the pathway of plant hormone signal transduction. An in vitro study showed that the transcriptional levels of ERF1B-like TF for hexokinase inhibitor and sucrose treatments were upregulated, being respectively 28.1- and 30.2-fold higher than in the control, suggesting that ERF1B-like TFs participate in the sugar-induced regulation of flavonoid biosynthesis. The results of yeast one-hybrid and dual-luciferase assays demonstrated that CsF3′H, encoding flavonoid 3′-hydroxylase, was the target flavonoid biosynthetic gene for CsERF1B-like TF. Our study identified the potential key regulators participating in the metabolism of sugars and flavonoids, providing new insights into the crosstalk between sugar metabolism and flavonoid biosynthesis in tea plants.