Browse Articles

Article|31 Jan 2022|OPEN
DgMYB2 improves cold resistance in chrysanthemum by directly targeting DgGPX1 
Xiaohan Yang1 , Yunchen Luo1 , Huiru Bai1 , Xin Li1 , Si Tang1 , Xiaoqin Liao1 , Lei Zhang1 and Qinglin Liu,1 ,
1Department of Ornamental Horticulture, Sichuan Agricultural University, 211 Huimin Road, Wenjiang District, Chengdu, Sichuan 611130, China
*Corresponding author. E-mail: 13854@sicau.edu.cn

Horticulture Research 9,
Article number: uhab028 (2022)
doi: https://doi.org/10.1093/hr/uhab028
Views: 28

Received: 24 Jun 2021
Revised: 18 Jan 2022
Accepted: 03 Oct 2021
Published online: 31 Jan 2022

Abstract

MYB transcription factors play important roles in plant responses to cold stress, but the associated underlying mechanisms remain unclear. In this study, a cold-induced MYB transcription factor, DgMYB2, was isolated from chrysanthemum (Chrysanthemum morifolium Ramat). DgMYB2 was localized to the nucleus and exhibited transactivational activity. Overexpression of DgMYB2 improved cold tolerance in chrysanthemum, while cold tolerance in the antisense suppression lines decreased compared to that of the wild type. Additionally, electrophoretic mobility shift assays, chromatin immunoprecipitation, luciferase complementary imaging analysis, and dual-luciferase reporter gene detection experiments confirmed that DgMYB2 directly targets DgGPX1 and increases the activity of glutathione peroxidase to reduce the accumulation of reactive oxygen species, thereby improving cold resistance in chrysanthemum.