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Mini Review|14 Mar 2022|OPEN
Revisiting the dual pathway hypothesis of Chorismate production in plants
Joseph H. Lynch,1 ,
1Division of Plant and Soil Sciences, West Virginia University, Morgantown, WV 26506, USA
*Corresponding author. E-mail:

Horticulture Research 9,
Article number: uhac052 (2022)
Views: 176

Received: 31 Dec 2021
Accepted: 19 Feb 2022
Published online: 14 Mar 2022


The shikimate pathway, the seven enzymatic steps that synthesize chorismate from phosphoenolpyruvate and erythrose 4-phosphate, produces the last common precursor of the three aromatic amino acids. It is firmly established that all seven enzymes are present in plastids, and it is generally accepted that this organelle is likely the sole location for production of chorismate in plants. However, recently a growing body of evidence has provided support for a previous proposal that at least portions of the pathway are duplicated in the cytosol, referred to as the Dual Pathway Hypothesis. Here I revisit this obscure hypothesis by reviewing the findings that provided the original basis for its formulation as well as more recent results that provide fresh support for a possible extra-plastidial shikimate pathway duplication. Similarities between this possible intercompartmental metabolic redundancy and that of terpenoid metabolism are used to discuss potential advantages of pathway duplication, and the translational implications of the Dual Pathway Hypothesis for metabolic engineering are noted.