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Article|19 Feb 2022|OPEN
MdMYB10 affects nitrogen uptake and reallocation by regulating the nitrate transporter MdNRT2.4-1 in red-fleshed apple
Xin Liu1,4 ,† , Hao-Feng Liu1 ,† , Hong-Liang Li1 , Xiu-Hong An2 , Lai-Qing Song3 , Chun-Xiang You1 , Ling-Ling Zhao3 , and Yi Tian2 , , Xiao-Fei Wang,1 ,
1State Key Laboratory of Crop Biology, Shandong Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production, Shandong Green Fertilizer Technology Innovation Center, College of Horticulture Science and Engineering, Shandong Agricultural University, Taian 271018, Shandong, China
2National Engineering Research Center for Agriculture in Northern Mountainous Areas, Agricultural Technology Innovation Center in Mountainous Areas of Hebei Province, Hebei Agricultural University, Baoding, Hebei, China
3Yantai Academy of Agricultural Sciences, Yantai, Shandong, China
4Beijing Academy of Forestry and Pomology Sciences, Beijing, China
*Corresponding author. E-mail: zhaolingling122@163.com,taiyangheizi_129@163.com,xfwang2004@163.com
Both authors contributed equally to the study.

Horticulture Research 9,
Article number: uhac016 (2022)
doi: https://doi.org/10.1093/hr/uhac016
Views: 34

Received: 06 Oct 2021
Accepted: 04 Jan 2022
Published online: 19 Feb 2022

Abstract

Nitrate is the major nitrogen source for higher plants. In addition to serving as a nutrient, it is also a signaling molecule that regulates plant growth and development. Although membrane-bound nitrate transporter/peptide transporters (NRT/PTRs) have been extensively studied and shown to regulate nitrate uptake and movement, little is known about how these factors are regulated by the external nitrogen environment. Red-fleshed apple, whose coloration is determined by the transcription factor MdMYB10, had higher nitrate uptake efficiency than non-red-fleshed apple. Nitrate assimilation and utilization were higher in red-fleshed apple cultivars, and comparative transcriptome analysis showed that the expression of genes encoding the NRT2s was increased in red-fleshed apple. In vitro and in vivo experiments showed that MdMYB10 directly bound to the MdNRT2.4-1 promoter to transcriptionally activate its expression, resulting in enhanced nitrate uptake. MdMYB10 also controlled nitrate reallocation from old leaves to new leaves through MdNRT2.4-1. Overall, our findings provide novel insights into the mechanism by which MdMYB10 controls nitrate uptake and reallocation in apple, which facilitates adaptation to a low-nitrogen environment.