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Article|25 Feb 2022|OPEN
Identifying early metabolite markers of successful graft union formation in grapevine
Grégoire Loupit1 , , Josep Valls Fonayet2,3 , Sylvain Prigent2,4 , Duyen Prodhomme1 , Anne-Sophie Spilmont5 , Ghislaine Hilbert1 , Céline Franc3 , Gilles De Revel3 and Tristan Richard2,3 , Nathalie Ollat1 , Sarah Jane Cookson,1
1EGFV, University Bordeaux, Bordeaux Sciences Agro, INRAE, ISVV, F-33882 Villenave d’Ornon, France
2Bordeaux Metabolome Facility, MetaboHUB, PHENOME-EMPHASIS, Centre INRAE de Nouvelle Aquitaine - Bordeaux, av Edouard Bourlaux, 33140 Villenave d’Ornon, France
3University Bordeaux, Unité de recherche Œnologie, EA 4577, USC 1366 INRAE, ISVV, F33882 Villenave d’Ornon, France
4INRAE, University Bordeaux, UMR BFP, 33882 Villenave d’Ornon, France
5Institut Français de la Vigne et du Vin, Domaine de l’Espiguette, 30240 Le Grau-du-Roi, France
*Corresponding author. E-mail: gregoire.loupit@u-bordeaux.fr

Horticulture Research 9,
Article number: uhab070 (2022)
doi: https://doi.org/10.1093/hr/uhab070
Views: 402

Received: 13 Jul 2021
Revised: 08 Mar 2022
Accepted: 08 Nov 2021
Published online: 25 Feb 2022

Abstract

Grafting is an important horticultural technique used for many crop species. However, some scion/rootstock combinations are considered as incompatible due to poor graft union formation and subsequently high plant mortality. The early identification of graft incompatibility could allow the selection of non-viable plants before planting and would have a beneficial impact on research and development in the nursery sector. In general, visible phenotypes of grafted plants (size, root number, etc.) are poorly correlated with grafting success, but some studies have suggested that some polyphenols could be used as markers of graft incompatibility several months or years after grafting. However, much of the previous studies into metabolite markers of grafting success have not included all the controls necessary to unequivocally validate the markers proposed. In this study, we quantified 73 primary and secondary metabolites in nine hetero-grafts and six homo-grafted controls 33 days after grafting at the graft interface and in both the scion and rootstock woody tissues. Certain biomarker metabolites typical of a high stress status (such as proline, GABA and pallidol) were particularly accumulated at the graft interface of the incompatible scion/rootstock combination. We then used correlation analysis and generalized linear models to identify potential metabolite markers of grafting success measured one year after grafting. Here we present the first attempt to quantitatively predict graft compatibility and identify marker metabolites (especially asparagine, trans-resveratrol, trans-piceatannol and α-viniferin) 33 days after grafting, which was found to be particularly informative for homo-graft combinations.