Browse Articles

Article|01 Nov 2021|OPEN
Characterization of germline development and identification of genes associated with germline specification in pineapple
Lihua Zhao1,2, Liping Liu1, Yanhui Liu1, Xianying Dou1, Hanyang Cai1, Mohammad Aslam1,3, Zhimin Hou1, Xingyue Jin1, Yi Li1, Lulu Wang1, Heming Zhao1, Xiaomei Wang4, Adrien Sicard2 & Yuan Qin1,3,
1College of Life Science, State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Center for Genomics and Biotechnology, Fujian Agriculture and Forestry University, Fuzhou, China
2Department of Plant Biology, Swedish University of Agricultural Sciences, Uppsala BioCenter and Linnean Centre for Plant Biology, Uppsala, Sweden
3State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Key Lab of Sugarcane Biology, College of Agriculture, Guangxi University, Nanning, Guangxi, China
4Horticulture Research Institute, Guangxi Academy of Agricultural Sciences, Nanning Investigation Station of South Subtropical Fruit Trees, Ministry of Agriculture, Nanning, China

Horticulture Research 8,
Article number: 239 (2021)
doi: 10.1038/hortres.2021.239
Views: 12

Received: 02 Dec 2020
Revised: 01 Aug 2021
Accepted: 04 Aug 2021
Published online: 01 Nov 2021

Abstract

Understanding germline specification in plants could be advantageous for agricultural applications. In recent decades, substantial efforts have been made to understand germline specification in several plant species, including Arabidopsis, rice, and maize. However, our knowledge of germline specification in many agronomically important plant species remains obscure. Here, we characterized the female germline specification and subsequent female gametophyte development in pineapple using callose staining, cytological, and whole-mount immunolocalization analyses. We also determined the male germline specification and gametophyte developmental timeline and observed male meiotic behavior using chromosome spreading assays. Furthermore, we identified 229 genes that are preferentially expressed at the megaspore mother cell (MMC) stage during ovule development and 478 genes that are preferentially expressed at the pollen mother cell (PMC) stage of anther development using comparative transcriptomic analysis. The biological functions, associated regulatory pathways and expression patterns of these genes were also analyzed. Our study provides a convenient cytological reference for exploring pineapple germline development and a molecular basis for the future functional analysis of germline specification in related plant species.