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Article|01 Aug 2021|OPEN
Integrative genomics reveals paths to sex dimorphism in Salix purpurea L
Brennan Hyden1, Craig H. Carlson1, Fred E. Gouker1,2, Jeremy Schmutz3,4, Kerrie Barry3, Anna Lipzen3, Aditi Sharma3, Laura Sandor3, Gerald A. Tuskan5, Guanqiao Feng6, Matthew S. Olson6, Stephen P. DiFazio7 & Lawrence B. Smart1,
1Horticulture Section, School of Integrative Plant Science, Cornell University, Cornell AgriTech, Geneva, NY, USA
2Floral and Nursery Plants Research Unit, US National Arboretum, United States Department of Agriculture, Agricultural Research Service, Beltsville, MD, USA
3United States Department of Energy, Joint Genome Institute, Berkeley, CA, USA
4HudsonAlpha Institute for Biotechnology, Huntsville, AL, USA
5The Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN, USA
6Department of Biology, Texas Tech University, Lubbock, TX, USA
7Department of Biology, West Virginia University, Morgantown, WV, USA

Horticulture Research 8,
Article number: 170 (2021)
doi: 10.1038/hortres.2021.170
Views: 317

Received: 21 Mar 2021
Revised: 23 May 2021
Accepted: 01 Jun 2021
Published online: 01 Aug 2021

Abstract

Sex dimorphism and gene expression were studied in developing catkins in 159 F2 individuals from the bioenergy crop Salix purpurea, and potential mechanisms and pathways for regulating sex development were explored. Differential expression, eQTL, bisulfite sequencing, and network analysis were used to characterize sex dimorphism, detect candidate master regulator genes, and identify pathways through which the sex determination region (SDR) may mediate sex dimorphism. Eleven genes are presented as candidates for master regulators of sex, supported by gene expression and network analyses. These include genes putatively involved in hormone signaling, epigenetic modification, and regulation of transcription. eQTL analysis revealed a suite of transcription factors and genes involved in secondary metabolism and floral development that were predicted to be under direct control of the sex determination region. Furthermore, data from bisulfite sequencing and small RNA sequencing revealed strong differences in expression between males and females that would implicate both of these processes in sex dimorphism pathways. These data indicate that the mechanism of sex determination in Salix purpurea is likely different from that observed in the related genus Populus. This further demonstrates the dynamic nature of SDRs in plants, which involves a multitude of mechanisms of sex determination and a high rate of turnover.