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Article|01 Jul 2021|OPEN
The apple C2H2-type zinc finger transcription factor MdZAT10 positively regulates JA-induced leaf senescence by interacting with MdBT2
Kuo Yang1, Jian-Ping An1, Chong-Yang Li2, Xue-Na Shen1, Ya-Jing Liu1, Da-Ru Wang1, Xing-Long Ji1, Yu-Jin Hao1, & Chun-Xiang You1,
1National Key Laboratory of Crop Biology, Shandong Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-An, Shandong 271018, China
2National Key Laboratory of Crop Biology, College of Life Science, Shandong Agricultural University, Tai-An, Shandong 271018, China

Horticulture Research 8,
Article number: 159 (2021)
doi: 10.1038/hortres.2021.159
Views: 154

Received: 01 Feb 2021
Revised: 15 Apr 2021
Accepted: 26 Apr 2021
Published online: 01 Jul 2021

Abstract

Jasmonic acid (JA) plays an important role in regulating leaf senescence. However, the molecular mechanisms of leaf senescence in apple (Malus domestica) remain elusive. In this study, we found that MdZAT10, a C2H2-type zinc finger transcription factor (TF) in apple, markedly accelerates leaf senescence and increases the expression of senescence-related genes. To explore how MdZAT10 promotes leaf senescence, we carried out liquid chromatography/mass spectrometry screening. We found that MdABI5 physically interacts with MdZAT10. MdABI5, an important positive regulator of leaf senescence, significantly accelerated leaf senescence in apple. MdZAT10 was found to enhance the transcriptional activity of MdABI5 for MdNYC1 and MdNYE1, thus accelerating leaf senescence. In addition, we found that MdZAT10 expression was induced by methyl jasmonate (MeJA), which accelerated JA-induced leaf senescence. We also found that the JA-responsive protein MdBT2 directly interacts with MdZAT10 and reduces its protein stability through ubiquitination and degradation, thereby delaying MdZAT10-mediated leaf senescence. Taken together, our results provide new insight into the mechanisms by which MdZAT10 positively regulates JA-induced leaf senescence in apple.