Browse Articles

Article|01 Jul 2021|OPEN
Sex biased expression of hormone related genes at early stage of sex differentiation in papaya flowers
Juan Liu1,2, Li-Yu Chen1, Ping Zhou 1, Zhenyang Liao1, Hai Lin1, Qingyi Yu3,4 & Ray Ming5
1FAFU and UIUC Joint Center for Genomics and Biotechnology, Key Laboratory of Genetics, Breeding and Multiple Utilization of Crops, Ministry of Education; Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology; College of Life Science, Fujian Agriculture and Forestry University, Fuzhou 350002 Fujian, China
2Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Chinese Academy of Sciences, Wuhan Botanical Garden, Wuhan 430074, China
3Texas A&M AgriLife Research Center at Dallas, Texas A&M University System, Dallas, TX 75252, USA
4Department of Plant Pathology & Microbiology, Texas A&M University, College Station, TX 77843, USA
5Department of Plant Biology, School of Integrative Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA

Horticulture Research 8,
Article number: 147 (2021)
doi: 10.1038/hortres.2021.147
Views: 128

Received: 28 May 2019
Revised: 18 Apr 2021
Accepted: 26 Apr 2021
Published online: 01 Jul 2021

Abstract

Sex types of papaya are controlled by a pair of nascent sex chromosomes, but molecular genetic mechanisms of sex determination and sex differentiation in papaya are still unclear. We performed comparative analysis of transcriptomic profiles of male and female floral buds at the early development stage before the initiation of reproductive organ primordia at which there is no morphological difference between male and female flowers. A total of 1734 differentially expressed genes (DEGs) were identified, of which 923 showed female-biased expression and 811 showed male-biased expression. Functional annotation revealed that genes related to plant hormone biosynthesis and signaling pathways, especially in abscisic acid and auxin pathways, were overrepresented in the DEGs. Transcription factor binding motifs, such as MYB2, GAMYB, and AP2/EREBP, were enriched in the promoters of the hormone-related DEGs, and transcription factors with those motifs also exhibited differential expression between sex types. Among these DEGs, we also identified 11 genes in the non-recombining region of the papaya sex chromosomes and 9 genes involved in stamen and carpel development. Our results suggested that sex differentiation in papaya may be regulated by multiple layers of regulation and coordination and involved transcriptional, epigenetic, and phytohormone regulation. Hormones, especially ABA and auxin, transcription factors, and genes in the non-recombination region of the sex chromosome could be involved in this process. Our findings may facilitate the elucidation of signal transduction and gene interaction in sex differentiation of unisexual flowers in papaya.