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Article|04 Mar 2021|OPEN
The key cyclic electron flow protein PGR5 associates with cytochrome b6f, and its function is partially influenced by the LHCII state transition
Xinyi Wu1, Jianqiang Wu1, Yu Wang1, Meiwen He1, Mingming He1, Weikang Liu1, Sheng Shu1,2, Jin Sun2,1, & Shirong Guo1,2
1College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China
2Nanjing Agricultural University (Suqian) Academy of Protected Horticulture, Jiangsu, Suqian 223800, China

Horticulture Research 8,
Article number: 55 (2021)
doi: 10.1038/hortres.2021.55
Views: 259

Received: 07 May 2020
Revised: 30 Oct 2020
Accepted: 20 Nov 2020
Published online: 04 Mar 2021

Abstract

In plants and algae, PGR5-dependent cyclic electron flow (CEF) is an important regulator of acclimation to fluctuating environments, but how PGR5 participates in CEF is unclear. In this work, we analyzed two PGR5s in cucumber (Cucumis sativus L.) under different conditions and found that CsPGR5a played the dominant role in PGR5-dependent CEF. The results of yeast two-hybrid, biomolecular fluorescence complementation (BiFC), blue native PAGE, and coimmunoprecipitation (CoIP) assays showed that PGR5a interacted with PetC, Lhcb3, and PsaH. Furthermore, the intensity of the interactions was dynamic during state transitions, and the abundance of PGR5 attached to cyt b6f decreased during the transition from state 1 to state 2, which revealed that the function of PGR5a is related to the state transition. We proposed that PGR5 is a small mobile protein that functions when attached to protein complexes.