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Article|01 Nov 2020|OPEN
Genetic dissection of climacteric fruit ripening in a melon population segregating for ripening behavior
Lara Pereira1, Miguel Santo Domingo1, Valentino Ruggieri1,2, Jason Argyris1,2, Michael A. Phillips3, Guangwei Zhao4, Qun Lian5, Yongyang Xu4, Yuhua He4, Sanwen Huang5, Marta Pujol1,2 & Jordi Garcia-Mas1,2,
1Centre for Research in Agricultural Genomics (CRAG) CSIC-IRTA-UAB-UB, Edifici CRAG, Campus UAB, 08193 Cerdanyola, Barcelona, Spain
2IRTA (Institut de Recerca i Tecnologia Agroalimentàries), Edifici CRAG, Campus UAB, 08193 Cerdanyola, Barcelona, Spain
3Department of Biology, University of Toronto–Mississauga, Mississauga, ON L5L 1C6, Canada
4Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou, China
5Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China

Horticulture Research 7,
Article number: 187 (2020)
doi: 10.1038/hortres.2020.187
Views: 299

Received: 28 May 2020
Revised: 04 Aug 2020
Accepted: 16 Sep 2020
Published online: 01 Nov 2020

Abstract

Melon is as an alternative model to understand fruit ripening due to the coexistence of climacteric and non-climacteric varieties within the same species, allowing the study of the processes that regulate this complex trait with genetic approaches. We phenotyped a population of recombinant inbred lines (RILs), obtained by crossing a climacteric (Védrantais, cantalupensis type) and a non-climcteric variety (Piel de Sapo T111, inodorus type), for traits related to climacteric maturation and ethylene production. Individuals in the RIL population exhibited various combinations of phenotypes that differed in the amount of ethylene produced, the early onset of ethylene production, and other phenotypes associated with ripening. We characterized a major QTL on chromosome 8, ETHQV8.1, which is sufficient to activate climacteric ripening, and other minor QTLs that may modulate the climacteric response. The ETHQV8.1 allele was validated by using two reciprocal introgression line populations generated by crossing Védrantais and Piel de Sapo and analyzing the ETHQV8.1 region in each of the genetic backgrounds. A Genome-wide association study (GWAS) using 211 accessions of the ssp. melo further identified two regions on chromosome 8 associated with the production of aromas, one of these regions overlapping with the 154.1 kb interval containing ETHQV8.1. The ETHQV8.1 region contains several candidate genes that may be related to fruit ripening. This work sheds light into the regulation mechanisms of a complex trait such as fruit ripening.