Browse Articles

Article|01 Sep 2020|OPEN
The curvature of cucumber fruits is associated with spatial variation in auxin accumulation and expression of a YUCCA biosynthesis gene
Shengnan Li1, Chunhua Wang1,2, Xiuyan Zhou1, Dong Liu1, Chunhong Liu1, Jie Luan1, Zhiwei Qin1 & Ming Xin1,
1College of Horticulture and Landscape Architecture, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Northeast Agricultural University, Harbin 150030, China
2College of Horticulture, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Fujian Agriculture and Forestry University, Fuzhou 350002, China

Horticulture Research 7,
Article number: 135 (2020)
doi: 10.1038/hortres.2020.135
Views: 454

Received: 11 Dec 2019
Revised: 11 Jun 2020
Accepted: 13 Jun 2020
Published online: 01 Sep 2020

Abstract

Fruit curving lowers the commercial value of cucumber and leads to significant economic losses. The mechanism driving the abnormal curving of cucumber is largely unknown. Through our previous work, we discovered that 2 days post-anthesis (DPA) was the key time point at which various phenotypic and genotypic characteristics of cucumber fruits are determined. Here, we analyzed the transcriptome of the concave (C1) and convex (C2) sides of curved fruits at 2 DPA by Gene Ontology (GO) enrichment and functional pathway enrichment analyses and identified auxin as a putative factor influencing fruit curvature. Changes in the curve angle in the fruits and exogenous auxin treatment analyses showed that asymmetric auxin distribution induces fruit curving. Identification of differentially expressed genes (DEGs) related to auxin and qPCR validation showed that CsYUC10b had the most significant differential expression when both sides of the curved fruits were compared. Gene functional analysis showed that the transcript levels of CsYUC10b and the auxin concentration were even on both sides of the fruit in CsYUC10b-overexpressing plants, which in turn contributed to an equal rate of growth of both sides of cucumber fruits and resulted in a straight shape of the fruits. Thus, we conclude that CsYUC10b promotes the formation of straight cucumber fruits, with possible applications in the production and breeding of cucumber.