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Article|01 Jul 2020|OPEN
Regulation of flowering time in chrysanthemum by the R2R3 MYB transcription factor CmMYB2 is associated with changes in gibberellin metabolism
Lu Zhu1, Yunxiao Guan1, Yanan Liu1, Zhaohe Zhang1, Muhammad Abuzar Jaffar1, Aiping Song1, Sumei Chen1, Jiafu Jiang1, & Fadi Chen1,
1State Key Laboratory of Crop Genetics and Germplasm Enhancement, Key Laboratory of Landscaping, Ministry of Agriculture, College of Horticulture, Nanjing Agricultural University, Nanjing, China

Horticulture Research 7,
Article number: 96 (2020)
doi: 10.1038/hortres.2020.96
Views: 363

Received: 23 Aug 2019
Revised: 01 Apr 2020
Accepted: 06 Apr 2020
Published online: 01 Jul 2020

Abstract

The switch from vegetative growth to reproductive growth is a key event in the development of a plant. Here, the product of the chrysanthemum gene CmMYB2, an R2R3 MYB transcription factor that is localized in the nucleus, was shown to be a component of the switching mechanism. Plants engineered to overexpress CmMYB2 flowered earlier than did wild-type plants, while those in which CmMYB2 was suppressed flowered later. In both the overexpression and RNAi knockdown plants, a number of genes encoding proteins involved in gibberellin synthesis or signaling, as well as in the response to photoperiod, were transcribed at a level that differed from that in the wild type. Both yeast two-hybrid and bimolecular fluorescence complementation assays revealed that CmMYB2 interacts with CmBBX24, a zinc-finger transcription factor known to regulate flowering by its influence on gibberellin synthesis.