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Article|01 Apr 2020|OPEN
Lipidomic and transcriptomic analysis reveals reallocation of carbon flux from cuticular wax into plastid membrane lipids in a glossy “Newhall” navel orange mutant
Haoliang Wan;;Jingyu Zhang;;Zhuoran Li;;Yizhong He;;Xiaoliang Zhang;;Xiuxin Deng;; Yunjiang Cheng ;;Weiwei Wen1 , Hongbo Liu2 , Yi Lyu3 , Yariv Brotman4 and Alisdair R. Fernie,5
1Key Laboratory of Horticultural Plant Biology (MOE), College of Horticulture and Forestry Sciences, Huazhong Agricultural University, 430070, Wuhan, China
2National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, China
3Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Youyi Xilu 127, Xi’an, 710072, Shaanxi, China
4Department of Life Sciences, Ben-Gurion University of the Negev, Beersheba, Israel
5Max-Planck-Institute of Molecular Plant Physiology, Am Muehlenberg 1, 14476, Potsdam, Germany

Horticulture Research 7,
Article number: 41 (2020)
doi: https://doi.org/10.1038/s41438-020-0262-z
Views: 1091

Received: 26 Jul 2019
Revised: 26 Dec 2019
Accepted: 07 Feb 2020
Published online: 01 Apr 2020

Abstract

Both cuticle and membrane lipids play essential roles in quality maintenance and disease resistance in fresh fruits. Many reports have indicated the modification of alternative branch pathways in epicuticular wax mutants; however, the specific alterations concerning lipids have not been clarified thus far. Here, we conducted a comprehensive, time-resolved lipidomic, and transcriptomic analysis on the “Newhall” navel orange (WT) and its glossy mutant (MT) “Gannan No. 1”. The results revealed severely suppressed wax formation accompanied by significantly elevated production of 36-carbon plastid lipids with increasing fruit maturation in MT. Transcriptomics analysis further identified a series of key functional enzymes and transcription factors putatively involved in the biosynthesis pathways of wax and membrane lipids. Moreover, the high accumulation of jasmonic acid (JA) in MT was possibly due to the need to maintain plastid lipid homeostasis, as the expression levels of two significantly upregulated lipases (CsDAD1 and CsDALL2) were positively correlated with plastid lipids and characterized to hydrolyze plastid lipids to increase the JA content. Our results will provide new insights into the molecular mechanisms underlying the natural variation of plant lipids to lay a foundation for the quality improvement of citrus fruit.