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Article|01 Dec 2018|OPEN
A naturally occurring variation in the BrMAM-3 gene is associated with aliphatic glucosinolate accumulation in Brassica rapa leaves
Jifang Zhang1,2, Hui Wang1,3, Zhiyuan Liu1, Jianli Liang1, Jian Wu1, Feng Cheng1, Shiyong Mei2 & Xiaowu Wang1,
1Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, 100081 Beijing, China
2Institute of Southern Economic Crops, Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, 410205 Changsha, China
3College of Horticulture, Qingdao Agricultural University, 266109 Qingdao, China

Horticulture Research 5,
Article number: 69 (2018)
doi: 10.1038/hortres.2018.69
Views: 570

Received: 17 Jan 2018
Revised: 28 May 2018
Accepted: 05 Jul 2018
Published online: 01 Dec 2018

Abstract

Glucosinolate profiles significantly vary among Brassica rapa genotypes. However, the molecular basis of these variations is largely unknown. In this study, we investigated a major quantitative trait locus (QTL) controlling aliphatic glucosinolate accumulation in B. rapa leaves. The QTL, which encompasses three tandem MAM genes and two MYB genes, was detected in two BC2DH populations. Among the five-candidate genes, only the expression level of BrMAM-3 (Bra013007) was significantly correlated with the accumulation of aliphatic glucosinolates in B. rapa leaves. We identified a naturally occurring insertion within exon 1 of BrMAM-3, which is predicted to be a loss-of-function mutation, as confirmed by qRT-PCR. We determined that the loss of function was associated with the low glucosinolate content in B. rapa accessions. Furthermore, overexpressing the BrMAM-3 gene resulted in an increase in total aliphatic glucosinolates in Arabidopsis transgenic lines. Our study provides insights into the molecular mechanism underlying the accumulation of aliphatic glucosinolates in B. rapa leaves, thereby facilitating in the manipulation of total aliphatic glucosinolate content in Brassica crops.