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Article|01 Sep 2018|OPEN
VlbZIP30 of grapevine functions in dehydration tolerance via the abscisic acid core signaling pathway
Mingxing Tu1,2, Xianhang Wang1,2, Yanxun Zhu1,2, Dejun Wang1,2, Xuechuan Zhang3, Ye Cui3, Yajuan Li1,2, Min Gao1,2, Zhi Li1,2, Yuejin Wang1,2 & Xiping Wang1,2,
1State Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, 712100 Yangling, Shaanxi, China
2Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Northwest A&F University, 712100 Yangling, Shaanxi, China
3Biomarker Technologies Corporation, 101300 Beijing, China

Horticulture Research 5,
Article number: 49 (2018)
doi: 10.1038/hortres.2018.49
Views: 770

Received: 14 Jan 2018
Revised: 06 May 2018
Accepted: 17 May 2018
Published online: 01 Sep 2018

Abstract

Drought stress limits the growth and development of grapevines, thereby reducing productivity, but the mechanisms by which grapevines respond to drought stress remain largely uncharacterized. Here, we characterized a group A bZIP gene from “Kyoho” grapevine, VlbZIP30, which was shown to be induced by abscisic acid (ABA) and dehydration stress. Overexpression of VlbZIP30 in transgenic Arabidopsis thaliana enhanced dehydration tolerance. Transcriptome analysis revealed that a major proportion of ABA-responsive and/or drought-responsive genes are transcriptionally regulated by VlbZIP30 during ABA or mannitol treatment at the cotyledon greening stage. We identified an A. thaliana G-box motif (CACGTG) and a potential grapevine G-box motif (MCACGTGK) in the promoters of the 39 selected A. thaliana genes upregulated in the transgenic plants and in the 35 grapevine homologs, respectively. Subsequently, using two grapevine-related databases, we found that 74% (23/31) and 84% (21/25) of the detected grapevine genes were significantly upregulated by ABA and drought stress, respectively, suggesting that these genes are involved in ABA or dehydration stress and may be regulated by VlbZIP30 in grapevine. We propose that VlbZIP30 functions as a positive regulator of dehydration-responsive signaling in the ABA core signaling pathway.