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Article|01 Sep 2018|OPEN
First genetic linkage map of Lathyrus cicera based on RNA sequencing-derived markers: Key tool for genetic mapping of disease resistance
Carmen Santos1, Nuno Felipe Almeida1, Mara Lisa Alves1, Ralf Horres2, Nicolas Krezdorn2, Susana Trindade Leitão1, Thaïs Aznar-Fernández3, Björn Rotter2, Peter Winter2, Diego Rubiales3 & Maria Carlota Vaz Patto1,
1Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Av. da República, Oeiras 2780-157, Portugal
2GenXPro GmbH, Frankfurt am Main D-60438, Germany
3Institute for Sustainable Agriculture, CSIC, Córdoba E-14004, Spain

Horticulture Research 5,
Article number: 45 (2018)
doi: 10.1038/hortres.2018.45
Views: 526

Received: 11 Jan 2018
Revised: 05 Mar 2018
Accepted: 30 Apr 2018
Published online: 01 Sep 2018

Abstract

The Lathyrus cicera transcriptome was analysed in response to rust (Uromyces pisi) infection to develop novel molecular breeding tools with potential for genetic mapping of resistance in this robust orphan legume species. One RNA-seq library each was generated from control and rust-inoculated leaves from two L. cicera genotypes with contrasting quantitative resistance, de novo assembled into contigs and sequence polymorphisms were identified. In toto, 19,224 SNPs differentiate the susceptible from the partially resistant genotype’s transcriptome. In addition, we developed and tested 341 expressed E-SSR markers from the contigs, of which 60.7% varied between the two L. cicera genotypes. A first L. cicera linkage map was created using part of the developed markers in a RIL population from the cross of the two genotypes. This map contains 307 markers, covered 724.2 cM and is organised in 7 major and 2 minor linkage groups, with an average mapping interval of 2.4 cM. The genic markers also enabled us to compare their position in L. cicera map with the physical position of the same markers mapped on Medicago truncatula genome, highlighting a high macrosyntenic conservation between both species. This study provides a large new set of genic polymorphic molecular markers with potential for mapping rust resistances. It represents the first step towards genomics-assisted precision breeding in L. cicera.