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Article|31 Aug 2016|OPEN
Transcriptome analyses of the Dof-like gene family in grapevine reveal its involvement in berry, flower and seed development
Danielle Costenaro da Silva1 , Vítor da Silveira Falavigna1 , Marianna Fasoli2 , Vanessa Buffon3 , Diogo Denardi Porto4 , Georgios Joannis Pappas Jr5 , Mario Pezzotti6 , Giancarlo Pasquali1 and Luís Fernando Revers,3 ,
1Graduate Program in Cell and Molecular Biology, Center for Biotechnology, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS 91501-970, Brazil
2Dipartimento di Biotecnologie, Università degli Studi di Verona, Verona 37134, Italy Present address: E. & J. Gallo Winery, Modesto, CA 95354, USA
3Embrapa Uva e Vinho, Bento Gonçalves, RS 95701-008, Brazil
4Embrapa Semiárido, Petrolina, PE 56302-970, Brazil
5Department of Cell Biology, Universidade de Brasília, Brasília, DF 70910-900, Brazil
6Dipartimento di Biotecnologie, Università degli Studi di Verona, Verona 37134, Italy
*Corresponding author. E-mail: luis.revers@embrapa.br

Horticulture Research 3,
Article number: 42 (2016)
doi: https://doi.org/10.1038/hortres.2016.42
Views: 720

Received: 30 Jun 2016
Revised: 08 Aug 2016
Accepted: 09 Aug 2016
Published online: 31 Aug 2016

Abstract

The Dof (DNA-binding with one finger) protein family spans a group of plant transcription factors involved in the regulation of several functions, such as plant responses to stress, hormones and light, phytochrome signaling and seed germination. Here we describe the Dof-like gene family in grapevine (Vitis vinifera L.), which consists of 25 genes coding for Dof. An extensive in silico characterization of the VviDofL gene family was performed. Additionally, the expression of the entire gene family was assessed in 54 grapevine tissues and organs using an integrated approach with microarray (cv Corvina) and real-time PCR (cv Pinot Noir) analyses. The phylogenetic analysis comparing grapevine sequences with those of Arabidopsis, tomato, poplar and already described Dof genes in other species allowed us to identify several duplicated genes. The diversification of grapevine DofL genes during evolution likely resulted in a broader range of biological roles. Furthermore, distinct expression patterns were identified between samples analyzed, corroborating such hypothesis. Our expression results indicate that several VviDofL genes perform their functional roles mainly during flower, berry and seed development, highlighting their importance for grapevine growth and production. The identification of similar expression profiles between both approaches strongly suggests that these genes have important regulatory roles that are evolutionally conserved between grapevine cvs Corvina and Pinot Noir.