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Article|22 Jun 2016|OPEN
Relative quantification of phosphoproteomic changes in grapevine (Vitis vinifera L.) leaves in response to abscisic acid
Supakan Rattanakan1 , Iniga George2 , Paul A Haynes2 and Grant R Cramer,1 ,
1Department of Biochemistry and Molecular Biology, University of Nevada, Reno, Reno, NV, USA
2Department of Chemistry and Biomolecular Sciences, Macquarie University, North Ryde, New South Wales, Australia
*Corresponding author. E-mail: cramer@unr.edu

Horticulture Research 3,
Article number: 29 (2016)
doi: https://doi.org/10.1038/hortres.2016.29
Views: 686

Received: 01 Mar 2016
Revised: 16 May 2016
Accepted: 16 May 2016
Published online: 22 Jun 2016

Abstract

In a previous transcriptomic analysis, abscisic acid (ABA) was found to affect the abundance of a number of transcripts in leaves of Cabernet Sauvignon grapevines with roots that had been exposed to 10 μm ABA for 2 h. Other work has indicated that ABA affects protein abundance and protein phosphorylation as well. In this study we investigated changes in protein abundance and phosphorylation of Cabernet Sauvignon grapevine leaves. Protein abundance was assessed by both label-free and isobaric-label quantitive proteomic methods. Each identified common proteins, but also additional proteins not found with the other method. Overall, several thousand proteins were identified and several hundred were quantified. In addition, hundreds of phosphoproteins were identified. Tens of proteins were found to be affected in the leaf after the roots had been exposed to ABA for 2 h, more than half of them were phosphorylated proteins. Many phosphosites were confirmed and several new ones were identified. ABA increased the abundance of some proteins, but the majority of the proteins had their protein abundance decreased. Many of these proteins were involved in growth and plant organ development, including proteins involved in protein synthesis, photosynthesis, sugar and amino-acid metabolism. This study provides new insights into how ABA regulates plant responses and acclimation to water deficits.