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Article|16 Sep 2015|OPEN
Redox proteomics of tomato in response to Pseudomonas syringae infection
Kelly Mayrink Balmant1,2 , Jennifer Parker1,2 , Mi-Jeong Yoo1 and Ning Zhu1 , Craig Dufresne3 , Sixue Chen,1,2,4 ,
1Department of Biology, Genetics Institute, University of Florida, Gainesville, FL, USA
2Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, FL, USA
3Thermo Fisher Scientific, 1400 Northpoint Parkway, West Palm Beach, FL, USA
4Interdisciplinary Center for Biotechnology Research, University of Florida, Gainesville, FL, USA
*Corresponding author. E-mail: schen@ufl.edu

Horticulture Research 2,
Article number: 43 (2015)
doi: https://doi.org/10.1038/hortres.2015.43
Views: 1018

Received: 20 Apr 2015
Revised: 20 Aug 2015
Accepted: 20 Aug 2015
Published online: 16 Sep 2015

Abstract

Unlike mammals with adaptive immunity, plants rely on their innate immunity based on pattern-triggered immunity (PTI) and effector-triggered immunity (ETI) for pathogen defense. Reactive oxygen species, known to play crucial roles in PTI and ETI, can perturb cellular redox homeostasis and lead to changes of redox-sensitive proteins through modification of cysteine sulfhydryl groups. Although redox regulation of protein functions has emerged as an important mechanism in several biological processes, little is known about redox proteins and how they function in PTI and ETI. In this study, cysTMT proteomics technology was used to identify similarities and differences of protein redox modifications in tomato resistant (PtoR) and susceptible (prf3) genotypes in response to Pseudomonas syringae pv tomato (Pst) infection. In addition, the results of the redox changes were compared and corrected with the protein level changes. A total of 90 potential redox-regulated proteins were identified with functions in carbohydrate and energy metabolism, biosynthesis of cysteine, sucrose and brassinosteroid, cell wall biogenesis, polysaccharide/starch biosynthesis, cuticle development, lipid metabolism, proteolysis, tricarboxylic acid cycle, protein targeting to vacuole, and oxidation–reduction. This inventory of previously unknown protein redox switches in tomato pathogen defense lays a foundation for future research toward understanding the biological significance of protein redox modifications in plant defense responses.